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Noradrenaline modulates mechanically evoked responses in the rat spinal dorsal horn: an in vivo patch-clamp study

Authors Sonohata M, Doi A, Yasaka T, Uta D, Mawatari M, Yoshimura M

Received 23 July 2018

Accepted for publication 14 February 2019

Published 17 April 2019 Volume 2019:12 Pages 1269—1278

DOI https://doi.org/10.2147/JPR.S181210

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Dr Michael A Überall


Motoki Sonohata,1 Atsushi Doi,2 Toshiharu Yasaka,3 Daisuke Uta,4 Masaaki Mawatari,1 Megumu Yoshimura5,6

1Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga, Japan; 2Department of Physical Therapy, Kumamoto Health Science University, Kumamoto, Japan; 3Department of Immunology, Graduate School of Medical and Dental SciencesKagoshima University, Kagoshima, Japan; 4Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan; 5Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; 6Nakamura Hospital, Nogata, Fukuoka, Japan

Purpose: We investigated the effects of noradrenaline (NA) on physiologically evoked synaptic responses of substantia gelatinosa (SG) neurons using anesthetized animals.
Methods: Male Sprague–Dawley rats (6–8 weeks, 200–300 g, n=21) were anesthetized. The lumbar spinal cord was exposed from L3 to L5; subsequently, the rats were fixed to a stereotaxic apparatus. The electrode was advanced at an angle of 30–45 degrees into the SG using a micromanipulator. We recorded excitatory post-synaptic currents (EPSC). Under these conditions, innocuous or noxious mechanical stimuli were applied to the receptive field of the ipsilateral hindlimb with or without NA, respectively.
Results: NA (50 μM) pre-application induced three types of responses for pinch-evoked EPSCs. The number of neurons showing inhibition, facilitation, and no-effect was 15 (71.4%), 2 (9.5%), and 4 (19%), respectively (n=21). Pre-treatment with NA also induced three different types of responses for puff-evoked EPSC (n=21). The number of neurons showing inhibition, facilitation, and no-effect was 9 (42.9%), 9 (42.9%), and 3 (14.2%), respectively. Further, there was a significant difference in the rate distribution (inhibition, facilitation, and no change) between puff- and pinch-evoked responses.
Conclusion: Our present data indicate that NA acts on noxious and innocuous mechanical transmission in the SG. Considering the distinct sensory inputs to the SG, the different actions of NA on the transmission of sensory information imply that NA exerts its analgesic effects in a manner more complicated than previously believed.

Keywords: noradrenaline, in vivo patch-clamp technique, touch, pain, spinal dorsal horn


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