Neuroprotective effect and mechanism of baicalin on Parkinson’s disease model induced by 6-OHDA
Authors Tu L, Wu ZY, Yang XL, Zhang Q, Gu R, Wang Q, Tian T, Yao H, Qu X, Tian JY
Received 20 February 2018
Accepted for publication 28 June 2018
Published 3 January 2020 Volume 2019:15 Pages 3615—3625
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Professor Wai Kwong Tang
Li Tu,1 Zhuo-Yu Wu,2 Xiu-Lin Yang,3 Qian Zhang,3 Ran Gu,3 Qian Wang,2 Tian Tian,2 Huan Yao,3 Xiang Qu,3 Jin-Yong Tian2,3
1Department of General Medical, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China; 2Department of Neurology, Guizhou Provincial People’s Hospital, Guiyang, Guizhou, China; 3Department of Emergency, Guizhou Provincial People’s Hospital, Guiyang, Guizhou, China
Correspondence: Jin-Yong Tian
Department of Emergency, Guizhou Provincial People’s Hospital, 83 Zhongshan East Road, Guiyang 550002, Guizhou, China
Tel +86 851 8593 7194
Objective: This research was aimed to investigate the effects of baicalin on 6-hydroxydopamine (6-OHDA)-induced rat model of Parkinson’s disease (PD) and the main mechanism of baicalin based on metabolomics.
Methods: The rat model of PD was induced by 6-OHDA. The protective effects of baicalin on rat model of PD were evaluated by open field test and rotarod test. The anti-PD efficacy of baicalin was evaluated by examining the morphologic changes of neurons and the level of monoamine neurotransmitters in the striatum, the number and morphology of tyrosine hydroxylase (TH)-positive neurons, and oxidative stress. Combined with metabolomics methods, the pharmacodynamic mechanism of baicalin on PD pathogenesis was also explored.
Results: Baicalin treatment improved the rod time and voluntary movement in rat model of PD (P<0.05) by the open field test and rotarod test. In addition, baicalin also protected from oxidative stress injury (P<0.05), and regulated the content of monoamine neurotransmitters dopamine, 3,4-dihydroxyphenylacetic acid, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid (P<0.05) and the number and morphology of TH-positive cells in 6-OHDA-induced PD model rats. By metabolomics, multivariate statistical analysis, and receiver operating characteristic curve analysis, we found that two metabolites N-acetyl aspartic acid and glutamic acid had a good diagnostic value. Quantitative analysis of metabolites showed a regulatory function of baicalin.
Conclusion: Baicalin has significant protective effect on 6-OHDA-induced PD rats, which may play a protective role through an antioxidant, promoting the release of neurotransmitters and regulating the metabolism of N-acetyl aspartate and glutamate.
Keywords: Parkinson’s disease, neurotransmitter, baicalin, metabolomics
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