Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Neurite outgrowth stimulatory effects of myco­synthesized AuNPs from Hericium erinaceus (Bull.: Fr.) Pers. on pheochromocytoma (PC-12) cells

Authors Raman J, Lakshmanan H, John P, Zhijian C, Periasamy V, David P, Naidu M, Sabaratnam V

Received 11 May 2015

Accepted for publication 18 July 2015

Published 18 September 2015 Volume 2015:10(1) Pages 5853—5863


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 4

Editor who approved publication: Prof. Dr. Thomas J Webster

Jegadeesh Raman,1 Hariprasath Lakshmanan,1 Priscilla A John,1,2 Chan Zhijian,3 Vengadesh Periasamy,3 Pamela David,1,4 Murali Naidu,1,4 Vikineswary Sabaratnam1,2

1Mushroom Research Centre, 2Institute of Biological Sciences, Faculty of Science, University of Malaya, 3Low Dimensional Materials Research Center (LDMRC), Department of Physics, Faculty of Science, 4Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

Background: Hericium erinaceus has been reported to have a wide range of medicinal properties such as stimulation of neurite outgrowth, promotion of functional recovery of axonotmetic peroneal nerve injury, antioxidant, antihypertensive, and antidiabetic properties. In recent years, the green synthesis of gold nanoparticles (AuNPs) has attracted intense interest due to the potential use in biomedical applications. The aim of this study was to investigate the effects of AuNPs from aqueous extract of H. erinaceus on neurite outgrowth of rat pheochromocytoma (PC-12) cells.
Methods: The formation of AuNPs was characterized by UV–visible spectrum, energy dispersive X-ray (EDX), field-emission scanning electron microscope (FESEM), transmission electron microscopy (TEM), particle size distribution, and Fourier transform-infrared spectroscopy (FTIR). Furthermore, the neurite extension study of synthesized AuNPs was evaluated by in vitro assay.
Results: The AuNPs exhibited maximum absorbance between 510 and 600 nm in UV–visible spectrum. FESEM and TEM images showed the existence of nanoparticles with sizes of 20–40 nm. FTIR measurements were carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the nanoparticles. The purity and the crystalline properties were confirmed by EDX diffraction analysis, which showed strong signals with energy peaks in the range of 2–2.4 keV, indicating the existence of gold atoms. The synthesized AuNPs showed significant neurite extension on PC-12 cells. Nerve growth factor 50 ng/mL was used as a positive control. Treatment with different concentrations (nanograms) of AuNPs resulted in neuronal differentiation and neuronal elongation. AuNPs induced maximum neurite outgrowth of 13% at 600 ng/mL concentration.
Conclusion: In this study, the AuNPs synthesis was achieved by a simple, low-cost, and rapid bioreduction approach. AuNPs were shown to have potential neuronal differentiation and stimulated neurite outgrowth. The water-soluble bioconstituents could be responsible for the neuroactivity. This is the first report for the biosynthesis of AuNPs using the hot aqueous extract of H. erinaceus.

Keywords: AuNPs, nanoparticles, Hericium erinaceus, PC-12, neurite outgrowth

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other article by this author:

Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells

Gurunathan S, Raman J, Malek SN, John PA, Vikineswary S

International Journal of Nanomedicine 2013, 8:4399-4413

Published Date: 15 November 2013

Readers of this article also read:

Nanoparticulated docetaxel exerts enhanced anticancer efficacy and overcomes existing limitations of traditional drugs

Choi J, Ko E, Chung HK, Lee JH, Ju EJ, Lim HK, Park I, Kim KS, Lee JH, Son WC, Lee JS, Jung J, Jeong SY, Song SY, Choi EK

International Journal of Nanomedicine 2015, 10:6121-6132

Published Date: 29 September 2015

Hepatotoxicity assessment of Mn-doped ZnS quantum dots after repeated administration in mice

Yang YJ, Lv SY, Yu BF, Xu S, Shen JM, Zhao T, Zhang HX

International Journal of Nanomedicine 2015, 10:5787-5796

Published Date: 15 September 2015

Endothelial cell-targeted pVEGF165 polyplex plays a pivotal role in inhibiting intimal thickening after vascular injury

Tian S, Cao D, Zou H, Bai F, Wang Z, Pan S, Feng M

International Journal of Nanomedicine 2015, 10:5751-5768

Published Date: 10 September 2015

Delivery of DNAzyme targeting aurora kinase A to inhibit the proliferation and migration of human prostate cancer

Xing Z, Gao S, Duan Y, Han H, Li L, Yang Y, Li Q

International Journal of Nanomedicine 2015, 10:5715-5727

Published Date: 9 September 2015

Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells

Kim JH, Kim HR, Lee BR, Choi ES, In SI, Kim EJ

International Journal of Nanomedicine 2015, 10:5513-5528

Published Date: 31 August 2015

Molecular targets in arthritis and recent trends in nanotherapy

Roy K, Kanwar RK, Kanwar JR

International Journal of Nanomedicine 2015, 10:5407-5420

Published Date: 26 August 2015

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010