Nanoscale hepatoprotective herbal decoction attenuates hepatic stellate cell activity and chloroform-induced liver damage in mice

Sherry Huang¹, Shu-Jen Chang², Miffy Yang³, Justin Jin-Ching Chen³, Walter H Chang^4
1Department of Biomedical Engineering, Chung Yuan Christian University, Chungli, Taiwan; ²School of Pharmacy, China Medical University, Taichung, Taiwan; ³Center for Nano Bioengineering, ⁴Department of Biomedical Engineering, Chung Yuan Christian University, Chungli, Taiwan

Background: San-Huang-Xie-Xin-Tang (SHXXT) decoction, a traditional Chinese medicine containing Rhei rhizome, Coptidis rhizome, and Scutellariae radix, is widely used in hepatoprotective therapy. However, preparation of the decoction requires addition of boiling water that causes loss of numerous effective components.
Methods: To improve the bioavailability of the decoction, nanoscale SHXXT was developed. Chloroform-induced liver injury and hepatic stellate cell activity in mice were used to demonstrate the hepatoprotective characteristics of nanoscale SHXXT decoction.
Results: Liver/body weight ratio and serum aspartate and alanine aminotranferase levels were recovered by the nanoscale SHXXT. TIMP-1 gene expression was inhibited and MMP-2 gene expression was accelerated in activated hepatic stellate cells.
Conclusion: Nanoscale SHXXT decoction prepared in room temperature water could have preserved hepatoprotective ability. The results of this study indicate that nanoscale SHXXT could be extracted easily. The simple preparation of this herbal decoction is more convenient and energy-efficient.

Keywords: Chinese herb medicine, San-Huang-Xie-Xin-Tang, nanoparticle, liver fibrosis