Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo

Marta Grodzik¹, Ewa Sawosz¹, Mateusz Wierzbicki¹, Piotr Orlowski¹, Anna Hotowy², Tomasz Niemiec¹, Maciej Szmidt³, Katarzyna Mitura⁴, André Chwalibog^2
1Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland; ²Department of Basic Animal and Veterinary Science, University of Copenhagen, Copenhagen, Denmark; ³Division of Histology and Embryology, Warsaw University of Life Sciences, Warsaw, Poland; ⁴Department of Biomedical Engineering, Koszalin University of Technology, Koszalin, Poland

Abstract: The objective of the study was to determine the effect of carbon nanoparticles produced by different methods on the growth of brain tumor and the development of blood vessels. Glioblastoma multiforme cells were cultured on the chorioallantoic membrane of chicken embryo and after 7 days of incubation, were treated with carbon nanoparticles administered in ovo to the tumor. Both types of nanoparticles significantly decreased tumor mass and volume, and vessel area. Quantitative real-time polymerase chain reaction analysis showed downregulated fibroblast growth factor-2 and vascular endothelial growth factor expression at the messenger ribonucleic acid level. The present results demonstrate antiangiogenic activity of carbon nanoparticles, making them potential factors for anticancer therapy.

Keywords: cancer, nanoparticle, embryo, angiogenesis, FGF-2, VEGF