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Naa10p Enhances Chemosensitivity to Cisplatin in Oral Squamous Cell Carcinoma Cells

Authors Sun L, Wang K, Peng L, Zhang J, Yang J, Zhao J, Xu J, Zheng J, Zeng Y

Received 11 December 2020

Accepted for publication 2 February 2021

Published 22 February 2021 Volume 2021:13 Pages 1843—1851


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Sanjeev Srivastava

Lichun Sun,1,* Kaixin Wang,2,* Lu Peng,1 Jinfang Zhang,2 Jie Yang,3 Juan Zhao,1 Jiang Xu,1 Jun Zheng,1 Yan Zeng1

1Department of Stomatology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, People’s Republic of China; 2Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong, People’s Republic of China; 3Department of Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Yan Zeng; Jun Zheng
Department of Stomatology & Key Laboratory of Xinjiang Endemic and Ethnic Disease, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, People’s Republic of China
Tel +86 18997732862
; +86 18997737192

Background: This study aimed to investigate the function and underlying molecular mechanism of N-α-acetyltransferase 10 protein (Naa10p) in cisplatin (CDDP) chemosensitivity in oral squamous cell carcinoma (OSCC).
Methods: Salivary Naa10p levels in 76 OSCC patients undergoing CDDP-based chemotherapy were detected using enzyme-linked immunosorbent assay. Quantitative real-time polymerase chain reaction and Western blot were used to examine the expression of Naa10p in constructed CDDP-resistant OSCC cell (Cal-27/CDDP) lines and nude mouse model. In addition, the tumor volume and weight of nude mice were analyzed. Lentiviral system was employed to establish and identify OSCC cell lines with stable Naa10p interference or overexpression. MTT assay was used for drug sensitivity analysis. P-gp and Bcl-2 expression levels were tested by Western blot.
Results: Higher salivary Naa10p expression was present in the complete response/partial response group (n=46) compared to the stable disease/progressive disease group (n=30) in OSCC patients receiving chemotherapy treatment. Naa10p expression was down-regulated in Cal-27/CDDP cells and tissues. Naa10p overexpression significantly reduced the expression level of drug-resistant molecules. Naa10p was related to CDDP resistance and enhanced CDDP sensitivity in OSCC according to drug sensitivity analysis and nude mouse model experiments.
Conclusion: Naa10p plays a tumor suppressor gene role and is associated with CDDP resistance in OSCC. It can enhance CDDP sensitivity in OSCC and may be a potential target for OSCC chemotherapy.

Keywords: N-α-acetyltransferase 10 protein, oral squamous cell carcinoma, cisplatin, chemosensitivity

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