Back to Journals » Infection and Drug Resistance » Volume 13

Molecular Epidemiology of Carbapenemase-Producing Pseudomonas aeruginosa Isolated from an Iranian University Hospital: Evidence for Spread of High-Risk Clones

Authors Ohadian Moghadam S, Afshar D, Nowroozi MR, Behnamfar A, Farzin A

Received 13 March 2020

Accepted for publication 17 May 2020

Published 3 June 2020 Volume 2020:13 Pages 1583—1592

DOI https://doi.org/10.2147/IDR.S253756

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony


Solmaz Ohadian Moghadam,1 Davoud Afshar,2 Mohammad Reza Nowroozi,1 Amir Behnamfar,1 Amirreza Farzin1

1Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran; 2Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran

Correspondence: Solmaz Ohadian Moghadam
Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Tel/ Fax +98 21 66 43 77 25
Email s-ohadian@sina.tums.ac.ir

Purpose: Given the importance of treatment failure due to multidrug-resistant (MDR) strains, studies on population structure of these organisms are necessary to improve control strategies. Accordingly, the current study aimed to determine the prevalence of carbapenem-resistant P. aeruginosa (CRPA) at a teaching referral hospital in Iran and to analyze their molecular clonality by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) for epidemiological purposes.
Methods: In this study, modified Hodge test (MHT) and double-disk synergy test (DDST) were used for carbapenemase production and metallo-β-lactamases (MBLs) screening, respectively. All P. aeruginosa isolates were tested for antimicrobial resistance. Moreover, MBL genes (blaIMP, blaVIM, blaSPM, blaNDM) were detected by multiplex PCR assay.
Results: Among 68 P. aeruginosa clinical isolates, 38 (55.88%) isolates were CRPA. Antibiotic susceptibility testing revealed that most of these isolates were MDR. PFGE analyses showed 5 common types and 27 single types among CRPA isolates. MLST analysis revealed three major clusters (MLST-sequence types (STs): 235, 357, and 861) among them. The 30 non-CRPA isolates corresponded mainly to MLST-STs 253, 360, and 446.
Conclusion: Our results showed that internationally distributed MLST-STs with widely genomic diversity have spread in our hospital, and clonal expansion of MDR strains of P. aeruginosa was described as well.

Keywords: carbapenem-resistant P. aeruginosa, CRPA, carbapenemase, metallo-β-lactamases, MBLs, multidrug-resistant, MDR, Pseudomonas aeruginosa, multilocus sequence typing, MLST, pulsed-field gel electrophoresis, PFGE

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]