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Molecular Detection of the mcr Genes by Multiplex PCR

Authors Liu J, Zhang Z, Feng Y, Hu H, Yu Y, Qiu L, Liu H, Guo Z, Huang J, Du C, Qiu J

Received 31 March 2020

Accepted for publication 16 September 2020

Published 12 October 2020 Volume 2020:13 Pages 3463—3468


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Héctor Mora-Montes

Jiayang Liu1 ,* Zhiyuan Zhang1 ,* Yuyang Feng,1 Huimin Hu,1 Yu Yu,1 Lihao Qiu,1 Hongtao Liu,1 Zhimin Guo,2 Jing Huang,2 Chongtao Du,1 Jiazhang Qiu1

1Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, People’s Republic of China; 2Department of Clinical Laboratory, The First Hospital of Jilin University, Changchun 130021, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jiazhang Qiu; Chongtao Du
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, People’s Republic of China

Background: The emergence and prevalence of plasmid-mediated colistin-resistant bacterial strains in recent years have raised great concerns in clinical medicine. It is urgently needed to develop a cheaper, faster, simpler, sensitive, and specific molecular detection method to identify and monitor the dissemination of the transferable resistant determinants.
Methods and Results: Herein, eight pairs of primers were designed to set up a multiplex PCR method for the rapid and efficient determination of reported mcr genes. This assay can give results within 85 min (35 min for amplification and 50 min for electrophoresis). We validated the feasibility of this assay by testing the presence of mcr genes in 60 colistin-resistant isolates.
Conclusion: Our multiplex PCR technique exhibits remarkable advantages in the light of clear identification, efficiency of amplification, as well as the time consuming for detection, and thus could be useful for the surveillance and epidemiological research of plasmid-mediated colistin resistance, particularly for the under-resourced laboratories.

Keywords: multiplex PCR, colistin resistance, mcr genes

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