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Molecular characterization of the pilS2 gene and its association with the frequency of Pseudomonas aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island

Authors Bahramian A, Khoshnood S, Shariati A, Doustdar F, Salimi Chirani A, Heidary M

Received 1 October 2018

Accepted for publication 12 December 2018

Published 11 January 2019 Volume 2019:12 Pages 221—227

DOI https://doi.org/10.2147/IDR.S188527

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony


Aghil Bahramian,1,2 Saeed Khoshnood,3,4 Aref Shariati,5 Farahnoosh Doustdar,2 Alireza Salimi Chirani2 Mohsen Heidary5,6

1Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 3Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 4Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 5Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; 6Student Research Committee, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran

Introduction: Pseudomonas aeruginosa is the most common opportunistic pathogen associated with a broad range of infections, including cystic fibrosis, ocular, otitis media, and burn infections. The aim of this study was to show the frequency of the pilS2 gene, and its association with P. aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island among P. aeruginosa strains.
Methods: The samples were collected from patients with cystic fibrosis, ocular, otitis media, and burn infections between January 2016 and November 2017. DNA was extracted using the DNA extraction kit and was used for PCR assay. PCR with 4 primer-pairs including 976 F/PAPI-1R, 4542 F/intF, SojR/4541 F, and intF/sojR was performed to identify PAPI-1. pKLC102 was detected using three other primer-pairs including cp10F/ cp10R, cp44F/cp44R, and cp97F/cp97R.
Results: A total of 112 P. aeruginosa isolates were collected from patients with cystic fibrosis (36), burn (20), otitis media (26), and ocular (30) infections. The results of PCR showed that pilS2 gene was identified in 96 (85%) strains. PAPI-1–attB integration was detected among 38 (33.9%) isolates and the circular form of PAPI-1 detected among 17 (14%) isolates. In addition, 79 (70.5%) strains were found to be positive for pKLC102.
Conclusion: We found that the majority of the isolates may be susceptible to transfer this significant island and the related element pKLC102 into recipient isolates lacking the island owing to high association of the PilS2 pilus with the islands in the studied strains. It is anticipated that strains isolated from burn and eye with the highest rate of PilS2, PAPI-1, and pKLC102 association have a high level of antibiotic resistance.

Keywords: Pseudomonas aeruginosa, pilS2, cystic fibrosis, pKLC102, PAPI-1

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