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Mitochondrial DNA in Fresh versus Frozen Embryo Culture Media of Polycystic Ovarian Syndrome Patients Undergoing Invitro Fertilization: A Possible Predictive Marker of a Successful Pregnancy

Authors Sayed GA, Al-Sawaf HA, Al-Sawaf AH, Saeid M, Maged A, Ibrahim IH

Received 26 September 2020

Accepted for publication 14 December 2020

Published 11 January 2021 Volume 2021:14 Pages 27—38

DOI https://doi.org/10.2147/PGPM.S284064

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Martin Bluth


Video abstract presented by Ghadir A Sayed.

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Ghadir A Sayed,1 Hussein A Al-Sawaf,1 Ahmed H Al-Sawaf,2 Mohamed Saeid,3 Ahmed Maged,2 Iman Hassan Ibrahim4

1Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt; 2Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University, Cairo, Egypt; 3Ganna Center for IVF, Cairo, Egypt; 4Department of Biochemistry, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Correspondence:Ghadir A Sayed
Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo 12111, Egypt
Tel +201111826634
Fax +2028609117
Email ghadir-ali@eru.edu.eg

Purpose: Frozen embryos transfer (ET) may improve the live-birth and reduce rates of ovarian hyperstimulation in polycystic ovary syndrome (PCOS) patients. Morphological criteria are the classical way for embryo selection, yet recently, many biochemical and genetic markers have been developed. This study aimed to compare fresh and frozen ET using the mtDNA/gDNA ratio of embryo secretome and the possibility of using this ratio as a predictive marker of PCOS pregnancy rate.
Subjects and Methods: One hundred PCOS patients undergoing IVF were chosen according to Rotterdam criteria and divided into two groups. Group I (50 with fresh ET), group II (50 with frozen ET), and otherwise 33 apparently healthy women as a control group with fresh ET. We then carried out absolute quantification of embryo culture media mtDNA and gDNA by real-time PCR.
Results: mtDNA/gDNA ratio was significantly low in PCOS embryo culture media in comparison with control. Additionally, while the mtDNA/gDNA ratio was significantly high in pregnant PCOS embryo culture media, it was high, though not statistically significant, in the fresh ET than frozen ET group. mtDNA/gDNA ratio sensitivity and specificity in PCOS embryo culture media as a predictive value of pregnancy rate were (86% and 96%, respectively).
Conclusion: mtDNA/gDNA ratio measurement in PCOS embryo culture media is a novel marker that can be clinically applied as a predictive value of the quality of the morphologically good embryo.

Keywords: frozen embryos transfer, fresh embryos transfer, mtDNA, polycystic ovary syndrome

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