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miRNA-543 promotes cell migration and invasion by targeting SPOP in gastric cancer

Authors Xu J, Wang F, Wang X, He Z, Zhu X

Received 2 January 2018

Accepted for publication 20 March 2018

Published 21 August 2018 Volume 2018:11 Pages 5075—5082

DOI https://doi.org/10.2147/OTT.S161316

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 3

Editor who approved publication: Dr Ingrid Espinoza


Junfei Xu,1,2 Feiran Wang,2 Xi Wang,3 Zhixian He,2 Xinguo Zhu1

1Department of General Surgery, First Affiliated Hospital, Soochow University, Suzhou, People’s Republic of China; 2Department of General Surgery, Affiliated Hospital, Nantong University, Nantong, People’s Republic of China; 3Medical College of Nantong University, Nantong, People’s Republic of China

Background/purpose: Given the emerging role of microRNA (miRNA) in cancer progression, we investigated the role and mechanism of miRNA-543 (miR-543) in gastric cancer (GC).
Materials and methods: Real-time quantitative polymerase chain reaction was conducted to quantify the expression of miR-543. Luciferase reporter assay was used to confirm the association between speckle-type POZ protein (SPOP) and 3'-UTR. Moreover, the role of miR-543 and SPOP in GC was detected using transwell assays. In addition, we investigated the function of miR-543 in the epithelial–mesenchymal transition (EMT) progression.
Results: miR-543 was upregulated in GC. We identified SPOP as a direct target of miR-543, revealing its expression to be inversely correlated with miR-543 expression in GC tissues. Moreover, restoration of SPOP could inhibit miR-543-induced GC cell migration and invasion, whereas downregulation of miR-543 inhibited cell migration and invasion, which was partly abrogated by SPOP knockdown. Furthermore, our data also showed that miR-543 induced EMT of GC cells.
Conclusion: Our results demonstrated that miR-543 functions as a crucial oncogenic miRNA in GC. It exerts strong tumor-promoting effects through targeting SPOP in GC cell migration and invasion.

Keywords: gastric cancer, miR-543, SPOP, EMT, invasion and migration

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