MiR-935 Promotes Clear Cell Renal Cell Carcinoma Migration and Invasion by Targeting IREB2
Authors Liu F, Chen Y, Chen B, Liu C, Xing J
Received 25 September 2019
Accepted for publication 16 December 2019
Published 30 December 2019 Volume 2019:11 Pages 10891—10900
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Alexandra R. Fernandes
This paper has been retracted.
Fei Liu, 1, 2 Yuedong Chen, 2 Bin Chen, 2 Chunxiao Liu, 1 Jinchun Xing 2
1Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, People’s Republic of China; 2Department of Urology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, People’s Republic of China
Correspondence: Chunxiao Liu
Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, People’s Republic of China
Department of Urology, The First Affiliated Hospital of Xiamen University, No. 55 Zhenhai Road, Xiamen 361003, People’s Republic of China
Tel +86-138 0608 9889
Purpose: Clear cell renal cell carcinoma (ccRCC) has the highest rate of metastasis and invasion in RCC and is the third most common adult urinary malignancy. miRNA may serve a critical role in human cancer development and progression, has been confirmed to play a pivotal role in RCC cell invasion and migration. Since miR‑935 had been verified to be an oncogene or tumor suppressor in various cancers, the role of miR‑935 in RCC was unclear.
Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was used to verify miR-935 expression. CCK-8 assay, wound healing assay and transwell assay were used to investigate the cell proliferation, migration and invasion of miR-935. Receiver operating characteristic (ROC) curve analysis was applied to discriminate different clinical classifications. Gain or loss of function approaches were used to investigate the cell proliferation, migration and invasion of miR-935 in vitro. Bioinformatics analysis and dual-luciferase reporter assay were used to identify the target of miR-935.
Results: MiR-935 had a higher expression level in RCC cells and cancer tissues. MiR-935 mimics promoted cell proliferation, migration and invasion, and miR-935 inhibitor inhibited cell inhibit malignancy of cancer cells. Bioinformatics analysis and dual-luciferase reporter assay identified iron-responsive element-binding protein 2 (IREB2) as a direct target of miR-935. qRT-PCR showed IREB2 expression was downregulated in ccRCC cancer tissues and high IREB2 expression had a longer overall survival (OS) and disease-free survival (DFS). Silencing IREB2 could reverse the function of miR-935 inhibitor on cell proliferation and metastasis in renal cancer cells.
Conclusion: The study indicated that miR-935 may act as an oncomiRNA and influenced migration and invasion progress of ccRCC by targeting IREB2. Oncogene miR-935 may be a molecular marker and uncover new strategies for ccRCC.
Keywords: miR-935, clear cell renal cell carcinoma, IREB2, migration, invasion
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