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MiR-582-5p Inhibits Bladder Cancer-Genesis by Suppressing TTK Expression

Authors Tian Y, Guan Y, Su Y, Luo W, Yang G, Zhang Y

Received 30 July 2020

Accepted for publication 30 September 2020

Published 20 November 2020 Volume 2020:12 Pages 11933—11944

DOI https://doi.org/10.2147/CMAR.S274835

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Chien-Feng Li


Yudong Tian,1 Yanbin Guan,2 Yang Su,1 Wenjian Luo,1 Guo Yang,1 Yu Zhang1

1Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, People’s Republic of China; 2School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450000, Henan, People’s Republic of China

Correspondence: Yudong Tian
Department of Urology, The First Affiliated Hospital of Zhengzhou University, No. 1 Longhu Zhonghuan Road, Jinshui District, Zhengzhou 450000, Henan, People’s Republic of China
Tel +86 150 371 83651
Email tian_yd95@163.com

Background: Bladder cancer (BC) refers to the malignant growth found in the cells and tissues of the urinary bladder. While many studies have researched the progression of BC, scientists are yet to fully understand the mechanism of BC. This research aimed to explore the role of miR-582-5p and its target gene TTK in BC pathogenesis.
Methods: The evaluation of miR-582-5p and TTK mRNA expression in BC tissues or cells was performed using qRT-PCR. TargetScan was then used to predict the binding site of miR-582-5p on TTK mRNA. Subsequently, dual-luciferase reporter and RNA pull-down assays were employed to validate the binding relationship between miR-582-5p and TTK mRNA. CCK-8, BrdU, flow cytometry, and caspase-3 activity assays were later conducted to evaluate the viability, proliferation, cell cycle, and apoptosis of BC cells.
Results: Investigations revealed that miR-582-5p was downregulated in BC tissues and cells. Meanwhile, miR-582-5p inhibited the viability and proliferation of BC cells while stimulating the apoptosis and cycle arrest of the cells. TTK, the target gene of miR-582-5p, was later found to be over-expressed in BC tissues and cells. TTK, however, was observed to exhibit an opposite effect on miR-582-5p. Simply put, it stimulated BC cell malignant phenotypes, and this stimulation could be directly reversed by miR-582-5p.
Conclusion: This research confirmed that miR-582-5p could restrain bladder carcinogenesis by inhibiting TTK expression.

Keywords: bladder cancer, miR-582-5p, TTK, proliferation, apoptosis

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