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miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression

Authors Cheng H, Dong H, Feng J, Tian H, Zhang H, Xu L

Received 29 January 2018

Accepted for publication 1 June 2018

Published 10 August 2018 Volume 2018:11 Pages 4711—4721

DOI https://doi.org/10.2147/OTT.S164052

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Geoffrey Pietersz


Hui Cheng,1,* Hui Dong,1,* Jianlin Feng,2 Hongyan Tian,1 Haixia Zhang,3 Lina Xu4

1Department of Pathology, The People’s Hospital of Shouguang, Weifang 262700, China; 2Department of Radiology, The People’s Hospital of Shouguang, Weifang 262700, China; 3Department of Radiotherapy, The People’s Hospital of Shouguang, Weifang 262700, China; 4Department of Respiratory Medicine, The People’s Hospital of Weifang, Weifang 262700, China

*These authors contributed equally to this work

Purpose: This article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma.
Materials and methods: miR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients’ tumor tissues and normal tissues were collected. Nthy-ori 3-1 and K1 cells were cultured. K1 cells were also transfected. Quantitative real-time polymerase chain reaction and Western blot were used to detect miR-497 and yes-associated protein 1 (YAP1) expression. Luciferase reporter assay was performed. MTT and Transwell assay were conducted to measure cells’ proliferation, migration and invasion. Immunofluorescence detection was used to detect YAP1-positive cells.
Results: miR-497 was downregulated, while YAP1 was upregulated in thyroid papillary carcinoma tissues and K1 cells (P<0.05). Compared with the negative control group, the OD495 value and the migrating and invasive cell number were significantly lower in miR-497 mimics group and significantly higher in miR-497 inhibitor group (P<0.05). YAP1 was the target gene of miR-497. Compared with blank group, the OD495 value and the migrating and invasive cell number were significantly lower in si-YAP1 group and significantly higher in miR-497 inhibitor group (P<0.05), while no significant difference was found between si-YAP1+inhibitors group and blank group in these indicators.
Conclusion:
miR-497 regulated the proliferation, migration and invasion of K1 cells by negatively regulating YAP1 expression.

Keywords: thyroid papillary carcinoma, miR-497, YAP1, proliferation, invasion

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