miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression
Authors Cheng H, Dong H, Feng J, Tian H, Zhang H, Xu L
Received 29 January 2018
Accepted for publication 1 June 2018
Published 10 August 2018 Volume 2018:11 Pages 4711—4721
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Prof. Dr. Geoffrey Pietersz
Hui Cheng,1,* Hui Dong,1,* Jianlin Feng,2 Hongyan Tian,1 Haixia Zhang,3 Lina Xu4
1Department of Pathology, The People’s Hospital of Shouguang, Weifang 262700, China; 2Department of Radiology, The People’s Hospital of Shouguang, Weifang 262700, China; 3Department of Radiotherapy, The People’s Hospital of Shouguang, Weifang 262700, China; 4Department of Respiratory Medicine, The People’s Hospital of Weifang, Weifang 262700, China
*These authors contributed equally to this work
Purpose: This article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma.
Materials and methods: miR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients’ tumor tissues and normal tissues were collected. Nthy-ori 3-1 and K1 cells were cultured. K1 cells were also transfected. Quantitative real-time polymerase chain reaction and Western blot were used to detect miR-497 and yes-associated protein 1 (YAP1) expression. Luciferase reporter assay was performed. MTT and Transwell assay were conducted to measure cells’ proliferation, migration and invasion. Immunofluorescence detection was used to detect YAP1-positive cells.
Results: miR-497 was downregulated, while YAP1 was upregulated in thyroid papillary carcinoma tissues and K1 cells (P<0.05). Compared with the negative control group, the OD495 value and the migrating and invasive cell number were significantly lower in miR-497 mimics group and significantly higher in miR-497 inhibitor group (P<0.05). YAP1 was the target gene of miR-497. Compared with blank group, the OD495 value and the migrating and invasive cell number were significantly lower in si-YAP1 group and significantly higher in miR-497 inhibitor group (P<0.05), while no significant difference was found between si-YAP1+inhibitors group and blank group in these indicators.
Conclusion: miR-497 regulated the proliferation, migration and invasion of K1 cells by negatively regulating YAP1 expression.
Keywords: thyroid papillary carcinoma, miR-497, YAP1, proliferation, invasion
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]