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miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma

Authors Liu C, Li M, Hu Y, Shi N, Yu H, Liu H, Lian H

Received 31 December 2015

Accepted for publication 23 March 2016

Published 19 May 2016 Volume 2016:9 Pages 2865—2871

DOI https://doi.org/10.2147/OTT.S103460

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Narasimha Reddy Parine

Peer reviewer comments 2

Editor who approved publication: Dr William Cho


Chengxia Liu, Ming Li, Yingbin Hu, Ning Shi, Haisheng Yu, Haiyan Liu, Haifeng Lian

Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People’s Republic of China

Abstract: Increasing evidence suggests that microRNAs are associated with many important biologic processes in carcinogenesis. Despite ample research revealing the dysregualtion of miR-486-5p in various cancers, little is known about the roles of miR-486-5p in colorectal carcinoma (CRC). In this study, we investigated the biological functions and molecular mechanisms of miR-486-5p in CRC growth and invasion, discussing the potential of using miR-486-5p as a biomarker for colorectal cancers. Our data revealed that miR-486-5p was significantly downregulated in CRC tissues compared with the paracancer tissue by quantitative real-time polymerase chain reaction and that miR-486-5p was downregulated to a greater extent in advanced stage cancer (stage III and IV) as compared to early stage cancer (stage I and II). Luciferase reporter assay verified that neuropilin-2 was a direct functional target of miR-486-5p in the CRC cells, and upregulation of miR-486-5p in CRC cells negatively correlated with the expression of neuropilin-2. Furthermore, overexpression of miR-486-5p inhibited the tumor growth and lymphangiogenesis in nude mice, which was reversed by overexpression of neuropilin-2. Taken together, our study suggested miR-486-5p might be a suppressor of CRC.

Keywords: miR-486-5p, colorectal carcinoma, neuropilin-2, tumor growth, lymphangiogenesis
 

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