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miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting UROC28 in hepatoma cells

Authors Kong Q, Han J, Deng H, Wu F, Guo S, Ye Z

Received 11 May 2018

Accepted for publication 20 August 2018

Published 4 October 2018 Volume 2018:11 Pages 6489—6503

DOI https://doi.org/10.2147/OTT.S173840

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 2

Editor who approved publication: Dr Arseniy Yuzhalin


Qinglei Kong,1,* Jianhua Han,1,* Hong Deng,2 Feilong Wu,1 Shaozhong Guo,2 Zhiqiang Ye1

1Department of Emergency, The Third Affiliated Hospital of Sun Yat-San University, Guangzhou 510630, China; 2Department of Infectious Disease and Clinical Laboratory, The Third Affiliated Hospital of Sun Yat-San University, Guangzhou 510630, China

*These authors contributed equally to this work

Objective: MicroRNA (miR)-431 plays an essential role in various human cancer types, particularly in the process of invasion. However, the function and mechanism of miR-431-5p in the invasion of hepatocellular carcinoma (HCC) remain undefined.
Methods: The expression levels of miR-431-5p and its potential target protein UROC28 in hepatocellular carcinoma cells and tissues were detected, and the levels of EMT markers in vivo and in vitro were also detected.
Results: MiR-431-5p was downregulated in HCC cell lines and tissues and associated with vascular invasion and tumor encapsulation. Furthermore, miR-431-5p was able to influence the epithelialto- mesenchymal transition (EMT) process in HCCLM3 and HUH7 cells. Mechanistically, it was discovered that miR-431-5p repressed invasion by targeting UROC28. Furthermore, miR-431-5p influenced the EMT markers in HCCLM3 and HUH7 cells by downregulating UROC28 expression. Similarly, in vivo assays confirmed that miR-431-5p upregulation in HCC cells remarkably inhibited tumor proliferation and influenced the EMT markers.
Conclusion: The current study has demonstrated that the miR-431-5p/UROC28 axis acts possible influence on the EMT in HCC. Upregulation of miR-431-5p could be an original approach for inhibiting tumor invasion.

Keywords: hepatocellular carcinoma, microRNA-431, UROC28, PBOV1, epithelial-to-mesenchymal transition

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