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MiR-381 functions as a tumor suppressor in colorectal cancer by targeting Twist1

Authors He X, Wei Y, Wang Y, Liu L, Wang W, Li N

Received 27 October 2015

Accepted for publication 16 December 2015

Published 7 March 2016 Volume 2016:9 Pages 1231—1239

DOI https://doi.org/10.2147/OTT.S99228

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Dekuang Zhao

Peer reviewer comments 4

Editor who approved publication: Dr Faris Farassati


Xinxin He, Yangnian Wei, Yong Wang, Ling Liu, Wen Wang, Nianfeng Li

Department of Hepatobiliary and Pancreatic Surgery, Xiangya Hospital, Central South University, Hunan, People’s Republic of China

Abstract: MiR-381 has been reported to be dysregulated in several human cancers. However, the function and mechanism of miR-381 in colorectal cancer (CRC) remains unclear. In the present study, the miR-381 expression was assessed in a cohort of 113 CRC specimens using real-time quantitative polymerase chain reaction (RTq-PCR), which demonstrated that miR-381 was significantly downregulated in CRC and correlated with distant metastasis and tumor, node, and metastasis (TNM) stage. Functional study revealed that restoration of miR-381 significantly inhibited the invasion, migration, and epithelial–mesenchymal transition (EMT) of CRC cells. Luciferase reporter assay validated that Twist1, an important EMT inducer, was a direct target of miR-381, and rescued Twist1 attenuated the function of miR-381 in CRC cells. Correlation analysis also revealed an inverse correlation between miR-381 and Twist1 expression levels in CRC specimens. Taken together, our results highlight the significance of miR-381/Twist1 interaction in the development and progression of CRC, and suggest that restoration of miR-381 may be a potential therapeutic strategy for the patients with CRC.

Keywords: colorectal cancer, miR-381, twist family bHLH transcription factor 1, Twist1, epithelial–mesenchymal transition,
 EMT

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