miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
Authors Zhang L, Chen Y, Wang H, Zheng X, Li C, Han Z
Received 7 May 2018
Accepted for publication 18 June 2018
Published 30 August 2018 Volume 2018:11 Pages 5293—5302
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 3
Editor who approved publication: Dr Federico Perche
Lansheng Zhang,1,2 Yanwei Chen,3 Hui Wang,3 Xia Zheng,2 Caihong Li,2 Zhengxiang Han3
1Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, People’s Republic of China; 2Department of Radiation Oncology, the Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, People’s Republic of China; 3Department of Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, People’s Republic of China
Background: The roles and related mechanism of miR-376a in breast cancer cell progression are unclear.
Methods: Kaplan-Meier plotter analysis was used to analyze the correlation between miR-376a and the overall survival (OS) of breast cancer patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect miR-376a level in breast cancer cells. Cell viability, transwell migration and invasion, and cell apoptosis were constructed to investigate the effects of miR-376a on breast cancer cells. Luciferase reporter and RNA immunoprecipitation (RIP) were used to explore the targeting of miR-376a on NRP-1.
Results: miR-376a expression was positively correlated with the overall survival of breast cancer patients, and significantly decreased in breast cancer cells. Functionally, miR-376a overexpression suppressed cell proliferation, migration and invasion, and promoted cells apoptosis. Additionally, miR-376a could directly target NRP-1 and exerted its effect through NRP-1.
Conclusion: miR-376a could suppress breast cancer cell progression via directly targeting NRP-1.
Keywords: miR-376a, NRP-1, breast cancer, Wnt/β-catenin migration
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