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MiR-216b suppresses colorectal cancer proliferation, migration, and invasion by targeting SRPK1

Authors Yao YF, Li QR, Wang H

Received 7 January 2018

Accepted for publication 6 February 2018

Published 23 March 2018 Volume 2018:11 Pages 1671—1681

DOI https://doi.org/10.2147/OTT.S161835

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 3

Editor who approved publication: Dr Ingrid Espinoza


Yanfen Yao,1 Qiaorong Li,1 Hong Wang2

1Department of Intensive Care Unit, Shandong Provincial Third Hospital, Jinan, People’s Republic of China; 2Department of General Surgery, Shandong Provincial Third Hospital, Jinan, People’s Republic of China

Background: MiR-216b has been reported to be involved in the development of some cancers, however, the role of miR-216b in colorectal cancer (CRC) remains unclear.
Purpose: This study aimed to investigate the mechanism underlying miR-216b-induced CRC development.
Methods: We detected the expression of miR-216b in 80 cases of CRC tissues and cell lines, and further analyzed the association between miR-216b and clinical pathological indicators as well as prognosis. In vitro, the miR-216b overexpression cell model was established for further functional assay.
Results: We demonstrated that miR-216b in CRC tissues and cell lines was markedly decreased compared with corresponding adjacent normal tissues and colonic mucosal epithelial cell line, and was obviously associated with the TNM stage, lymph node metastases, and poor overall survival as well as recurrence-free survival. Furthermore, we found that miR-216b inhibited cell proliferation, cell cycle, migration, and invasion by targeting 3'-UTR of SRPK1. Besides, SRPK1 over-expression reversed miR-216b-inhibited cell proliferation, migration and invasion, while SRPK1 inhibition aggravated these effects.
Conclusions: We identified that miR-216b suppresses colorectal cancer proliferation, migration and invasion by targeting SRPK1, which shed light on how miR-216b functions in CRC pathogenesis.

Keywords: MiR-216b, SRPK1, colorectal cancer, proliferation, migration, invasion

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