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miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5

Authors Cao H, Pan G, Tang S, Zhong N, Liu H, Zhou H, Peng Q, Zou Y

Received 6 December 2019

Accepted for publication 25 February 2020

Published 20 March 2020 Volume 2020:13 Pages 2369—2376

DOI https://doi.org/10.2147/OTT.S241366

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Geoffrey Pietersz


Hui Cao, Guihua Pan, Shiqiang Tang, Ni Zhong, Huake Liu, Haizhi Zhou, Qin Peng, Yongbin Zou

Department of Oncology, Chenzhou First People’s Hospital, Chenzhou, People’s Republic of China

Correspondence: Yongbin Zou
Department of Oncology, Chenzhou First People’s Hospital, 102 Luojiajing Road, Chenzhou 423000, People’s Republic of China
Tel +86-13507357355 Email gjnzhu888@163.com

Objective: Cervical carcinoma (CC) is a serious threat to women’s health and few effective therapeutic methods have been discovered. The purpose of this study is to explore the underlying mechanism of miR-145-5p in CC.
Methods: Bioinformatics methods were employed to analyze the gene expression data of CC from TCGA database. qRT-PCR was used to detect the expression of miR-145-5p and KLF5 in CC cells, and Western blot was employed for the examination of KLF5 protein level. The targeted relationship between miR-145-5p and KLF5 was verified by a dual-luciferase reporter assay. Moreover, CCK-8, wound healing assay and transwell invasion assay were used to analyze the effects of miR-145-5p overexpression or KLF5 silencing on the proliferation, migration and invasion of CC cells.
Results: miR-145-5p was shown to be down-regulated in CC tissues and cells, while KLF5 was up-regulated. miR-145-5p could bind to the complementary sequence within the wild type KLF5 3ʹUTR rather than the mutant one. In addition, miR-145-5p could effectively down-regulate KLF5, in turn inhibiting the proliferation, migration and invasion of CC cells.
Conclusion: miR-145-5p regulates the proliferation, migration and invasion of CC cells by targeting KLF5.

Keywords: cervical carcinoma, miR-145-5p, KLF5, proliferation, migration, invasion


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