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MicroRNA-562 negatively regulated c-MET/AKT pathway in the growth of glioblastoma cells

Authors Nie X, Su Z, Yan R, Yan A, Qiu S, Zhou Y

Received 6 September 2018

Accepted for publication 26 November 2018

Published 21 December 2018 Volume 2019:12 Pages 41—49

DOI https://doi.org/10.2147/OTT.S186701

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 3

Editor who approved publication: Dr Sanjay Singh


Xiaohu Nie, Zhongzhou Su, Renfu Yan, Ai Yan, Sheng Qiu, Yue Zhou

Department of Neurosurgery, Huzhou Central Hospital, Wuxing District, Huzhou, Zhejiang 313000, P.R. China

Background: MicroRNA-562 (miR-562) has been found to possess anti-cancer function in certain tumors. However, the function of miR-562 in glioblastoma (GBM) is still not fully understood.
Purpose: The aim at present study is to analyze the function of miR-562 and its possible target in GBM cells.
Patients and methods: In the present study, a total of 80 GBM samples and 16 adjacent noncancerous tissues were used to examine the expression of miR-562 and c-MET. In order to gain a deep insight into the molecular network of miR-562 and c-MET in GBM, the miR-562 mimic and inhibitor were transfected into two GBM cell lines (U251 and U87), respectively. Meanwhile, lentiviral vector was used to mediate overexpression of c-MET. Cell proliferation was examined via Cell Counting Kit-8 (CCK-8) assays. Meanwhile, cell apoptosis was analyzed by Annexin V-FTTC/PI staining assay.
Results: Our results indicated that the level of miR-562 was downregulated in GBM tissues and the expression of c-MET was upregulated in tumors. Cell proliferation analysis indicated that miR-562 was an anti-proliferation effector in GBM cells. Moreover, cell apoptosis analysis suggested the pro-apoptosis function of miR-562 in GBM cells.
Conclusion: Our results demonstrated that miR-562 negatively regulated the c-MET/AKT signal pathway. In addition, caspase-3 might also serve as another target for miR-562 in GBM cells. This research not only obtained a deep understanding of miR-562 but also provided evidence in terms of developing new prognostic biomarker for GBM.

Keywords: glioblastoma, miR-562, c-MET, AKT, p-AKT, caspase-3

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