Back to Journals » International Journal of Nanomedicine » Volume 11

Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells

Authors Alarifi S, Ali D, Alkahtani S

Received 6 November 2015

Accepted for publication 7 January 2016

Published 29 March 2016 Volume 2016:11 Pages 1253—1259

DOI https://doi.org/10.2147/IJN.S99995

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Alexander Kharlamov

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster

Saud Alarifi, Daoud Ali, Saad Alkahtani

Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia

Abstract: Chromium oxide nanoparticles (Cr2O3NPs) are widely used in polymers and paints. In the present study, we aimed to determine the toxicity of Cr2O3NPs in murine fibrosarcoma (L929) cells. The cytotoxicity of Cr2O3NPs was measured by MTT and neutral red uptake assays; Cr2O3NPs had significant cytotoxic effects on L929 cells. Enhancement of intracellular reactive oxygen species was observed in L929 cells after exposure to Cr2O3NPs. Cr2O3NPs produced caspase-3, indicating that exposure to Cr2O3NPs induced apoptosis. After exposure to Cr2O3NPs, the cellular glutathione level decreased and lipid peroxidation, superoxide dismutase, and catalase increased in a dose- and time-dependent manner. By using single-cell gel tests, we also observed increased DNA damage in a Cr2O3NP exposure-duration- and dose-dependent fashion. Cell toxicity and DNA damage may be useful biomarkers for determining the safety of Cr2O3NPs in human and animal health.

Keywords: Cr2O3NPs, L929 cells, MTT assay, oxidative stress
 

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

In vitro apoptotic and DNA damaging potential of nanobarium oxide

Alarifi S, Ali D, Al-Bishri W

International Journal of Nanomedicine 2016, 11:249-257

Published Date: 13 January 2016

Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells

Alarifi S, Ali D, Alkahtani S

International Journal of Nanomedicine 2015, 10:3751-3760

Published Date: 25 May 2015

In vivo DNA damaging and apoptotic potential of silver nanoparticles in Swiss albino mice

Al Gurabi MA, Ali D, Alkahtani S, Alarifi S

OncoTargets and Therapy 2015, 8:295-302

Published Date: 29 January 2015

Readers of this article also read:

Development of a robust pH-sensitive polyelectrolyte ionomer complex for anticancer nanocarriers

Lim CM, Youn YS, Lee KS, Hoang NH, Sim TH, Lee ES, Oh KT

International Journal of Nanomedicine 2016, 11:703-713

Published Date: 22 February 2016

Green synthesis of water-soluble nontoxic polymeric nanocomposites containing silver nanoparticles

Prozorova GF, Pozdnyakov AS, Kuznetsova NP, Korzhova SA, Emel’yanov AI, Ermakova TG, Fadeeva TV, Sosedova LM

International Journal of Nanomedicine 2014, 9:1883-1889

Published Date: 16 April 2014

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010