MC3T3-E1 osteoblast attachment and proliferation on porous hydroxyapatite scaffolds fabricated with nanophase powder

Ian O Smith¹, Laura R McCabe², Melissa J Baumann¹

¹Department of Chemical Engineering and Materials Science, ²Department of Physiology, Michigan State University, East Lansing, MI, USA

Abstract:  Porous bone tissue engineering scaffolds were fabricated using both nano hydroxyapatite (nano HA) powder (20 nm average particle size) and micro HA powder (10 µm average particle size), resulting in sintered scaffolds of 59 vol% porosity and 8.6±1.9 µm average grain size and 72 vol% porosity and 588±55 nm average grain size, respectively. Scanning electron microscopy was used to measure both the grain size and pore size. MC3T3-E1 osteoblast (OB) attachment and proliferation on both nano HA and micro HA porous scaffolds were quantified. As expected, OB cell number was greater on nano HA scaffolds compared with similarly processed micro HA scaffolds 5 days after seeding, while OB attachment did not appear greater on the nano HA scaffolds (p<0.05).

Keywords: hydroxyapatite, osteoblast, nanomaterial, porous, scaffold