Back to Browse Journals » International Journal of Nanomedicine » Volume 8 » Issue 1

Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway

Authors Yavlovich A, Viard M, Gupta K, Sine J, Vu M, Blumenthal R, Tata DB, Puri A

Received 9 March 2013

Accepted for publication 14 April 2013

Published 22 July 2013 Volume 2013:8(1) Pages 2575—2587

DOI https://dx.doi.org/10.2147/IJN.S44993

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Amichai Yavlovich,1,* Mathias Viard,1,2,* Kshitij Gupta,1,* Jessica Sine,1,* Mylinh Vu,1 Robert Blumenthal,1 Darrell B Tata,3 Anu Puri1,*

1Center for Cancer Research Nanobiology Program, National Cancer Institute, Frederick, MD, USA; 2Basic Science Program, SAIC-Frederick, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA; 3Centre for Devices and Radiological Health (CDRH)/Office of Science and Engineering Laboratories(OSEL)/Division of Physics, US Food and Drug Administration, White Oak, MD, USA

*These authors contributed equally to this work

Abstract: We recently reported on the physical characteristics of photo-triggerable liposomes containing dipalmitoylphosphatidylcholine (DPPC), and 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) carrying a photo agent as their payload. When exposed to a low-intensity 514 nm wavelength (continuous-wave) laser light, these liposomes were observed to release entrapped calcein green (Cal-G; Ex/Em 490/517 nm) but not calcein blue (Cal-B; Ex/Em 360/460 nm). In this study, we have investigated the mechanism for the 514 nm laser-triggered release of the Cal-G payload using several scavengers that are known specifically to inhibit either type I or type II photoreaction pathways. Liposomes containing DPPC:DC8,9PC: distearoylphosphatidylethanolamine (DSPE)-polyethylene glycol (PEG)-2000 (86:10:04 mole ratio) were loaded either with fluorescent (calcein) or nonfluorescent (3H-inulin) aqueous markers. In addition, a non-photo-triggerable formulation (1-palmitoyl-2-oleoyl phosphatidylcholine [POPC]:DC8,9PC:DSPE-PEG2000) was also studied with the same payloads. The 514 nm wavelength laser exposure on photo-triggerable liposomes resulted in the release of Cal-G but not that of Cal-B or 3H-inulin, suggesting an involvement of a photoactivated state of Cal-G due to the 514 nm laser exposure. Upon 514 nm laser exposures, substantial hydrogen peroxide (H2O2, ≈100 µM) levels were detected from only the Cal-G loaded photo-triggerable liposomes but not from Cal-B-loaded liposomes (≤10 µM H2O2). The Cal-G release from photo-triggerable liposomes was found to be significantly inhibited by ascorbic acid (AA), resulting in a 70%–80% reduction in Cal-G release. The extent of AA-mediated inhibition of Cal-G release from the liposomes also correlated with the consumption of AA. No AA consumption was detected in the 514 nm laser-exposed Cal B-loaded liposomes, thus confirming a role of photoactivation of Cal-G in liposome destabilization. Inclusion of 100 mM K3Fe(CN)6 (a blocker of electron transfer) in the liposomes substantially inhibited Cal-G release, whereas inclusion of 10 mM sodium azide (a blocker of singlet oxygen of type II photoreaction) in the liposomes failed to block 514 nm laser-triggered Cal-G release. Taken together, we conclude that low-intensity 514 nm laser-triggered release of Cal-G from photo-triggerable liposomes involves the type I photoreaction pathway.

Keywords: visible laser-triggered payload release, photo-agents, photopolymerizable phospholipids, photodynamic actions, reactive oxygen species

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other article by this author:

Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts

Sine J, Urban C, Thayer D, Charron H, Valim N, Tata DB, Schiff R, Blumenthal R, Joshi A, Puri A

International Journal of Nanomedicine 2015, 10:125-145

Published Date: 19 December 2014

Readers of this article also read:

Synthesis and characterization of low-toxicity N-caprinoyl-N-trimethyl chitosan as self-assembled micelles carriers for osthole

Hu XJ, Liu Y, Zhou XF, Zhu QL, Bei YY, You BG, Zhang CG, Chen WL, Wang ZL, Zhu AJ, Zhang XN, Fan YJ

International Journal of Nanomedicine 2013, 8:3543-3558

Published Date: 20 September 2013

Dextran-b-poly(L-histidine) copolymer nanoparticles for pH-responsive drug delivery to tumor cells

Hwang JH, Choi CW, Kim HW, Kim DH, Kwak TW, Lee HM, Kim CH, Chung CW, Jeong YI, Kang DH

International Journal of Nanomedicine 2013, 8:3197-3207

Published Date: 21 August 2013

Few-layer graphene sheets with embedded gold nanoparticles for electrochemical analysis of adenine

Biris AR, Pruneanu S, Pogacean F, Lazar MD, Borodi G, Ardelean S, Dervishi E, Watanabe F, Biris AS

International Journal of Nanomedicine 2013, 8:1429-1438

Published Date: 12 April 2013

Paclitaxel-Fe3O4 nanoparticles inhibit growth of CD138–  CD34– tumor stem-like cells in multiple myeloma-bearing mice

Yang C, Wang J, Chen D, Chen J, Xiong F, Zhang H, Zhang Y, Gu N, Dou J

International Journal of Nanomedicine 2013, 8:1439-1449

Published Date: 12 April 2013

Encapsulation of cisplatin in long-circulating and pH-sensitive liposomes improves its antitumor effect and reduces acute toxicity

Leite EA, Souza CM, Carvalho-Júnior AD, Coelho LG, Lana AM, Cassali GD, Oliveira MC

International Journal of Nanomedicine 2012, 7:5259-5269

Published Date: 9 October 2012

Degradable copolymer based on amphiphilic N-octyl-N-quatenary chitosan and low-molecular weight polyethylenimine for gene delivery

Liu CC, Zhu Q, Wu WH, Xu XL, Wang XY, Gao S, Liu KH

International Journal of Nanomedicine 2012, 7:5339-5350

Published Date: 8 October 2012

Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells

Wang K, Zhang T, Liu L, Wang XL, Wu P, Chen ZG, Ni C, Zhang JS, Hu FQ, Huang J

International Journal of Nanomedicine 2012, 7:4487-4497

Published Date: 13 August 2012

Multi-dye theranostic nanoparticle platform for bioimaging and cancer therapy

Singh AK, Hahn MA, Gutwein LG, Rule MC, Knapik JA, Moudgil BM, Grobmyer SR, Brown SC

International Journal of Nanomedicine 2012, 7:2739-2750

Published Date: 1 June 2012