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Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer

Authors Jia W, Zhang J, Ma F, Hao S, Li X, Guo R, Gao Q, Sun Y, Jia J, Li W

Received 25 January 2019

Accepted for publication 19 July 2019

Published 19 August 2019 Volume 2019:12 Pages 6653—6663

DOI https://doi.org/10.2147/OTT.S201832

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Federico Perche


Wenxiao Jia,*,1 Jiaqin Zhang,*,2 Fang Ma,1 Shengjie Hao,1 Xue Li,1 Ruiting Guo,1 Qianqian Gao,1 Yundong Sun,1 Jihui Jia,1 Wenjuan Li1

1Key Laboratory for Experimental Teratology of Chinese Ministry of Education, The Shandong Provincial Key Laboratory of Infection and Immunology, Department of Microbiology, School of Basic Medical Sciences, Shandong University, Jinan, People’s Republic of China; 2Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen, People’s Republic of China

Correspondence: Wenjuan Li
Key Laboratory for Experimental Teratology of Chinese Ministry of Education, The Shandong Provincial Key Laboratory of Infection and Immunology, Department of Microbiology, School of Basic Medical Sciences, Shandong University, No. 44, Wenhuaxi Road, Jinan, Shangdong Province, People’s Republic of China
Tel +86 5 318 838 2579
Fax +86 5 318 838 2502
Email wenjli@sdu.edu.cn

*These authors contributed equally to this work

Background: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC.
Method: Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells.
Results: We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro.
Conclusion: These findings may provide a potential therapeutic target for H. pylori-associated GC.

Keywords: long noncoding RNA, THAP9-AS1, gastric cancer, Helicobacter pylori
 

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