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LncRNA ZNF667-AS1 Promotes ABLIM1 Expression by Adsorbing microRNA-1290 to Suppress Nasopharyngeal Carcinoma Cell Progression

Authors Chen X, Huang Y, Shi D, Nie C, Luo Y, Guo L, Zou Y, Xie C

Received 10 January 2020

Accepted for publication 15 April 2020

Published 20 May 2020 Volume 2020:13 Pages 4397—4409

DOI https://doi.org/10.2147/OTT.S245554

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Leo Jen-Liang Su


Xi Chen,1,2 Yaping Huang,1 Dianyu Shi,2 Chuan Nie,3 Yiping Luo,4 Liangfen Guo,1 Yu Zou,1 Chun Xie5

1Department of Otorhinolaryngology, Guangdong Women and Children Hospital, Guang Zhou, Guangdong, 511400, People’s Republic of China; 2Department of Otorhinolaryngology, People’s Hospital of Longhua,Guangdong,People’s Republic of China; 3Department of Neonatology, Guangdong Women and Children Hospital, Guang Zhou 511400, Guangdong, People’s Republic of China; 4Department of Internal Medicine, Guangdong Women and Children Hospital, Guang Zhou 511400, Guangdong, People’s Republic of China; 5Department of Stomatology, People’s Hospital of Longhua, Shenzhen 518109, Guangdong, People’s Republic of China

Correspondence: Yu Zou; Chun Xie Email Zouyu10291@163.com; xiechun2002@163.com

Background: Recently, long non-coding RNAs (lncRNAs) have been elucidated to play essential roles in cancers, and the recognition of lncRNA expression patterns in nasopharyngeal carcinoma (NPC) may be helpful for indicating novel mechanisms underlying NPC carcinogenesis. Herein, we conducted this study to probe into the function of lncRNA ZNF667-AS1 in NPC progression with the involvement of microRNA-1290 (miR-1290) and actin-binding LIM protein 1 (ABLIM1).
Materials and Methods: In silico analysis screened differentially expressed genes and miRNAs in NPC and predicted potential mechanisms. ZNF667-AS1 expression was detected in NPC tissues and cells. The gain-and-loss function assays were performed to explore the effects of lncRNA ZNF667-AS1 and miR-1290 in NPC cell biological behaviors. In vivo experiments were further conducted to confirm the in vitro results.
Results: In silico analysis predicted that ZNF667-AS1 was diminished in NPC, which may downregulate ABLIM1 through sponging miR-1290. ZNF667-AS1 was poorly expressed in NPC tissues and cells, and overexpression of ZNF667-AS1 inhibited growth of NPC cells. ZNF667-AS1 competitively bound with miR-1290, thereby upregulating ABLIM1. miR-1290 resulted in the promotion of NPC cell progression by suppressing ABLIM1. Overexpression of ZNF667-AS1 or suppression of miR-1290 inhibited tumorigenicity of NPC cells in vivo.
Conclusion: This study highlights that lncRNA ZNF667-AS1 promotes ABLIM1 expression by sponging miR-1290 to suppress NPC cell progression.

Keywords: nasopharyngeal carcinoma, lncRNA ZNF667-AS1, ABLIM1, microRNA-1290, competing endogenous RNA

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