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LncRNA WT1-AS Downregulates Survivin by Upregulating miR-203 in Papillary Thyroid Carcinoma

Authors Le F, Luo P, Ouyang Q, Zhong X

Received 24 September 2019

Accepted for publication 17 December 2019

Published 20 January 2020 Volume 2020:12 Pages 443—449

DOI https://doi.org/10.2147/CMAR.S232294

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Rudolph Navari


Fei Le, 1 Ping Luo, 2 Qian Ouyang, 3 Xiaoming Zhong 4

1Department of Head and Neck Surgery, Jiangxi Province Cancer Hospital, Nanchang City, Jiangxi Province, 330029, People’s Republic of China; 2Department of Surgical Oncology, Nanchang Third Hospital Surgical Oncology, Nanchang City, Jiangxi Province, 330002, People’s Republic of China; 3Department of Intensive Medicine, Jiangxi Province Cancer Hospital, Nanchang City, Jiangxi Province, 330029, People’s Republic of China; 4Department of Tumor Radiotherapy, Jiangxi Province Cancer Hospital, Nanchang City, Jiangxi Province, 330029, People’s Republic of China

Correspondence: Xiaoming Zhong
Department of Tumor Radiotherapy, Jiangxi Province Cancer Hospital, No. 519 Beijing East Road, Nanchang City, Jiangxi Province 330029, People’s Republic of China
Tel +86 15979192617
Email nlsxhwdccm24@163.com

Objective: This study aimed to assessment the functions of lncRNA WT1-AS in papillary thyroid carcinoma (PTC).
Methods: Expression levels of WT1-AS in PTC and non-tumor tissues from 66 PTC patients were measured and compared by performing qPCR and paired t test, respectively. Cell proliferation (CCK-8) assay was performed to evaluate the effects of the overexpression of WT1-AS, miR-203 and survivin on the proliferation of IHH-4 (a human PTC cell line) cells.
Results: We found that WT1-AS was significantly downregulated in PTC and associated with clinical stages. In PTC tissues, WT1-AS was negatively correlated with survivin but positively correlated with miR-203. In PTC cells, WT1-AS overexpression led to significantly upregulated miR-203 and downregulated survivin. MiR-203 overexpression failed to affect WT1-AS but downregulated survivin. Cell proliferation assay showed that overexpression of WT1-AS and miR-203 led to decreased, while survivin overexpression led to increased proliferation of PTC cells. In addition, survivin overexpression attenuated the effects of WT1-AS and miR-203 overexpression.
Conclusion: Therefore, WT1-AS may downregulate survivin by upregulating miR-203 in PTC to inhibit cancer cell proliferation.

Keywords: WT1-AS, papillary thyroid carcinoma, survivin, miR-203

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