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LncRNA TP73-AS1 Activates TGF-β1 to Promote the Migration and Invasion of Colorectal Cancer Cell

Authors Li M, Jin Y, Li Y

Received 23 August 2019

Accepted for publication 26 November 2019

Published 16 December 2019 Volume 2019:11 Pages 10523—10529

DOI https://doi.org/10.2147/CMAR.S228490

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Professor Bilikere Dwarakanath


Mingli Li,1 Yuanyuan Jin,2 Yuanzhi Li3

1Department of Chinese traditional medicine, Jining University, Qufu City, Shandong Province, 273155, People’s Republic of China; 2The First People’s Hospital of Yuzhong County, Lanzhou City, Gansu Province 730100, People’s Republic of China; 3Department of Anorectal Hemorrhoid Fistula, The Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province 646000, People’s Republic of China

Correspondence: Yuanzhi Li
Department of Anorectal Hemorrhoid Fistula, The Affiliated Hospital of Southwest Medical University, No. 25 Taiping Street, Luzhou City, Sichuan Province 646000, People’s Republic of China
Tel +86-15982134672
Email tw25369@163.com

Purpose: LncRNA TP73-AS1 has been demonstrated to promote the developments of several types of human cancer. However, its role in colorectal cancer (CRC) is unknown.
Methods: All CRC patients (n=70, 40 males and 30 females, 38 to 66 years’ old, 52.1 ± 5.3 years’ old) in this study were enrolled in the Affiliated Hospital of Southwest Medical University from July 2012 to January 2014. Cells, vectors, and transient transfections, RT-qPCR, western-blotting, as well as measurements of cell migration and invasion abilities were carried out during the research.
Results: In the present study, we found that TP73-AS1 was upregulated in CRC tissues compared with adjacent non-CRC tissues in CRC patients. Upregulation of TP73-AS1 was closely correlated with poor prognosis. TGF-β1 was also upregulated in CRC tissues and positive correlated with TP73-AS1. TP73-AS1 overexpression caused upregulated TGF-β1 in CRC cells, while TGF-β1 overexpression showed no significant effect on TP73-AS1. TP73-AS1 and TGF-β1 overexpressions caused enhanced migration and invasion of CRC cells. TGF-β inhibitor treatment caused suppressed migration and invasion of CRC cells and attenuated effects of TP73-AS1 and TGF-β1 overexpression.
Conclusion: Therefore, TP73-AS1 may inactivate TGF-β1 to inhibit the migration and invasion of CRC cells.

Keywords: colorectal cancer, lncRNA TP73-AS1, TGF-β1, prognosis

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