LncRNA PLAC2 Positively Regulates CDK2 to Promote Ovarian Carcinoma Cell Proliferation
Authors He Y, Wei L, Zhang S, Liu H, Fang F, Li Y
Received 18 December 2019
Accepted for publication 17 May 2020
Published 12 July 2020 Volume 2020:12 Pages 5713—5720
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Sanjeev Srivastava
Yuanqi He,1 Liqun Wei,1 Shihong Zhang,1,2 Haining Liu,1 Fang Fang,1 Yue Li1
1Department of Gynaecology and Obstetrics, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, Shandong Province, 264200, People’s Republic of China; 2Department of Gynaecology and Obstetrics, Affiliated Hospital of Beihua University, Jilin City, Jilin Province 132000, People’s Republic of China
Correspondence: Shihong Zhang
Department of Gynecology and Obstetrics, Weihai Municipal Hospital,Cheeloo College of Medicine, Shandong University, 70 Heping Road, Huancui District, Weihai, Shandong Province 264200, People’s Republic of China
Background: PLAC2 has been reported to participate in glioma, but its role in ovarian carcinoma (OC) is unclear. This study investigated the role of lncRNA PLAC2 in OC.
Methods: A 5-year follow-up study of 64 patients was carried out in Weihai Municipal Hospital after the admission of patients. A total of 64 OC patients were selected from 178 OC patients admitted in the aforementioned hospital from August 2011 to January 2014. Cell transfections, cell cycle analysis, cell proliferation assay and Western blot were carried out during the research.
Results: The expression levels of PLAC2 and CDK2 were both upregulated in OC and they were positively correlated. During the 5-year follow-up, patients with high levels of PLAC2 and CDK2 showed significantly lower overall survival rate. In OC cells, overexpression of PLAC2 resulted in upregulated, while silencing of PLAC2 resulted in downregulated expression of CDK2. Cell proliferation assay showed that overexpression of PLAC2 resulted in increased, while silencing of PLAC2 resulted in decreased proliferation rate of OC cells. In addition, overexpression of CDK2 attenuated the effects of silencing of PLAC2.
Conclusion: PLAC2 positively regulates CDK2 to promote OC cell proliferation.
Keywords: ovarian carcinoma, CDK2, PLAC2, survival
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