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LncRNA LHFPL3-AS1 Promotes Oral Squamous Cell Carcinoma Growth and Cisplatin Resistance Through Targeting miR-362-5p/CHSY1 Pathway

Authors Li J, Xu X, Zhang D, Lv H, Lei X

Received 24 December 2020

Accepted for publication 8 March 2021

Published 31 March 2021 Volume 2021:14 Pages 2293—2300

DOI https://doi.org/10.2147/OTT.S298679

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Tohru Yamada


Jiandong Li, Xiaohu Xu, Dandan Zhang, Han Lv, Xin Lei

Department of Stomatology, Shenzhen Longhua District Central Hospital, Shenzhen, 518000, People’s Republic of China

Correspondence: Jiandong Li
Department of Stomatology, Shenzhen Longhua District Central Hospital, Shenzhen, 518000, People’s Republic of China
Email [email protected]

Background: Oral squamous cell carcinoma (OSCC) is a common oral cancer. The current study aims to elucidate the potential roles of long noncoding RNA (lncRNA) LHFPL3-AS1 in OSCC development.
Methods: Gene expression was measured by qRT-PCR in tumor tissues and cell lines. Loss-of-function assays were performed to analyze the effects of LHFPL3-AS1 on malignant behaviors. Bioinformatics analysis was conducted to explore the downstream signaling pathway of LHFPL3-AS1 in OSCC.
Results: LHFPL3-AS1 was highly expressed in OSCC tissues and cell lines. LHFPL3-AS1 was upregulated in cisplatin-resistant tumor cell lines. LHFPL3-AS1 level was correlated with survival rate. LHFPL3-AS1 knockdown suppressed OSCC proliferation, migration and invasion. LHFPL3-AS1 downregulation reduced cisplatin resistance of OSCC cells. LHFPL3-AS1 was the competing endogenous RNA (ceRNA) for miR-194-5p to enhance CHSY1 expression.
Conclusion: LHFPL3-AS1/miR-362-5p/CHSY1 signaling pathway plays essential roles in regulating OSCC development and cisplatin resistance.

Keywords: oral squamous cell carcinoma, LHFPL3-AS1, miR-362-5p, CHSY1

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