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LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis

Authors Chen T, Gao F, Yang T, Li H, Li Y, Ren H, Chen M

Received 3 January 2020

Accepted for publication 17 May 2020

Published 9 June 2020 Volume 2020:12 Pages 4379—4390

DOI https://doi.org/10.2147/CMAR.S244573

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Tian-jun Chen,1,* Fei Gao,1,2,* Tian Yang,1 Hong Li,1 Yang Li,1 Hui Ren,1 Ming-wei Chen1

1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, People’s Republic of China; 2Ultrasound Department, Huashan Central Hospital of Xi’an, Xi’an, Shaanxi 710043, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Ming-wei Chen
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277, Yanta West Road, Xi’an, Shaanxi, People’s Republic of China
Tel +86 29-85323888
Email wosyishen66@163.com

Background: Lung adenocarcinoma (ADC) is a major form of lung cancer, which is a main cause of global cancer-related death in male and female patients. LncRNAs are implicated in tumor development. However, the functions and mechanisms of the LncRNA HOTAIRM1 in ADC are not known.
Materials and Methods: Here, the downregulated HOTAIRM1 in ADC was selected by TCGA analysis. Subsequently, qRT-PCR, CCK-8, EdU, cell apoptosis, cell cycle and cell invasion assays were utilized for evaluating the roles of HOTAIRM1 in ADC. Finally, we explored the mechanism of HOTAIRM1 in ADC.
Results: HOTAIRM1 expression was considerably decreased in ADC tissues. The knockdown of HOTAIRM1 promoted the cell cycle, growth, and invasion of ADC. Moreover, HOTAIRM1 competitively bound miR-498 to regulate the expression of WWOX.
Conclusion: HOTAIRM1 suppressed the proliferation and invasion of ADC cells via the modulation of miR-498/WWOX axis. This finding suggested that it might be clinically valuable as a biomarker for ADC. Furthermore, the findings suggest LncRNA HOTAIRM1 as a candidate therapeutic target in ADC.

Keywords: HOTAIRM1, lung adenocarcinoma, miR-498, WWOX, cell proliferation

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