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LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo

Authors Chen L, Yang H, Xiao Y, Tang X, Li Y, Han Q, Fu J, Yang Y, Zhu Y

Received 13 October 2015

Accepted for publication 5 May 2016

Published 4 July 2016 Volume 2016:9 Pages 4075—4087

DOI https://doi.org/10.2147/OTT.S98203

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Prof. Dr. Haijun Zhang

Peer reviewer comments 2

Editor who approved publication: Dr Faris Farassati


Video abstract presented by Long Chen.

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Long Chen,1,2,* Huixin Yang,1,* Yanbin Xiao,3,* Xiaoxia Tang,4 Yuqian Li,1 Qiaoqiao Han,1 Junping Fu,3 Yuye Yang,1 Yuechun Zhu1

1Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 2PET/CT Center, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 3Department of Orthopaedics, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 4Department of Pharmacy, the Second Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China

*These authors contributed equally to this work

Abstract:
The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo.

Keywords: lncRNA, GAS5, melanoma, metastasis, gelatinase

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