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LncRNA FOXP4-AS1 Is Involved in Cervical Cancer Progression via Regulating miR-136-5p/CBX4 Axis

Authors Zhao J, Yang T, Li L

Received 10 December 2019

Accepted for publication 12 February 2020

Published 19 March 2020 Volume 2020:13 Pages 2347—2355

DOI https://doi.org/10.2147/OTT.S241818

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Gaetano Romano


Juan Zhao, Ting Yang, Long Li

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, People’s Republic of China

Correspondence: Long Li
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277, West Yantan Road, Xi’an 710061, People’s Republic of China
Email dr_lilong@sina.com

Introduction: Cervical cancer (CC) is a major health threat to women worldwide. Long non-coding RNA (lncRNA) has been reported to play crucial roles in regulating carcinogenesis, including CC.
Methods: In this work, levels of lncRNA forkhead box P4 antisense RNA 1 (FOXP4-AS1) in CC cell lines and normal cell lines were analyzed with quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) method. Effects of FOXP4-AS1 on CC cellular behaviors including proliferation, migration, and invasion were explored. Bioinformatic prediction tools and luciferase activity reporter assay were conducted to explore the downstream molecules for FOXP4-AS1.
Results: We found FOXP4-AS1 expression was significantly higher in CC cell lines than in normal cell line. Functionally, force FOXP4-AS1 expression increased CC cell proliferation, migration, and invasion, while FOXP4-AS1 knockdown caused opposite effects. Mechanistically, we found FOXP4-AS1 acts as competing endogenous RNA (ceRNA) for microRNA-136-5p (miR-136-5p) to regulate chromobox 4 (CBX4) expression.
Discussion: These findings indicated FOXP4-AS1 plays an oncogenic role in CC, which may provide novel therapeutic biomarker against CC.

Keywords: FOXP4-AS1, miR-136-5p, CBX4, cervical cancer

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