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LncRNA FOXD2-AS1 Regulates miR-25-3p/Sema4c Axis To Promote The Invasion And Migration Of Colorectal Cancer Cells

Authors Zhang M, Jiang X, Jiang S, Guo Z, Zhou Q, He J

Received 25 August 2019

Accepted for publication 15 October 2019

Published 19 December 2019 Volume 2019:11 Pages 10633—10639

DOI https://doi.org/10.2147/CMAR.S228628

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Antonella D'Anneo


Mengyan Zhang,1,* Xiang Jiang,2,* Sumei Jiang,3 Zhongying Guo,4 Qinfeng Zhou,5 Jingdong He1

1Department of Oncology, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an City, Jiangsu Province 223300, People’s Republic of China; 2Courage Pancreas Surgical, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an City, Jiangsu Province 223300, People’s Republic of China; 3Ultrasonic Department, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an City, Jiangsu Province 223300, People’s Republic of China; 4Department of Pathology, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an City, Jiangsu Province 223300, People’s Republic of China; 5Department of Laboratory Medicine, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province 215600, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jingdong He
Department Of Oncology, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, No. 6 Beijing West Road, Huaiyin District, Huai’an City, Jiangsu Province 223300, People’s Republic of China
Tel +86-0517-84907287
Email hejingdong66@yeah.net

Purpose: Although the roles of lncRNA FOXD2-AS1 have been investigated in many types of cancers including colorectal cancer (CRC), its functionality remains to be further investigated. Analysis of the TCGA data set revealed that FOXD2-AS1 was up-regulated in CRC tissues. This study aimed to analyze the function of FOXD2-AS1 in CRC.
Methods: FOXD2-AS1 expression was detected by qPCR. A 5-year follow-up study was performed to analyze the prognostic value of FOXD2-AS1 for CRC. Overexpression experiments were performed to analyze the interactions among FOXD2-AS1, miR-25-3p and Sema4C. Transwell assays were performed to analyze cell invasion and migration.
Results: In this study, we further confirmed the up-regulation of FOXD2-AS1 in CRC patients and showed that high FOXD2-AS1 level predicted poor survival. Bioinformatics analysis showed that miR-25-3p may bind FOXD2-AS1, while over-expression experiments showed no effects on each other’s expression. Instead, FOXD2-AS1 over-expression led to the up-regulate Sema4C, which is a target of miR-25-3p. Transwell assay showed that FOXD2-AS1 and Sema4C over-expression led to the increased invasion and migration rates of CRC cells. MiR-25-3p plays the opposite role and attenuated the effects of FOXD2-AS1 and Sema4C over-expression.
Conclusion: FOXD2-AS1 may regulate the miR-25-3p/Sema4C axis to promote the invasion and migration of CRC cells.

Keywords: colorectal cancer, FOXD2-AS1, miR-25-3p, Sema4C, survival

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