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LncRNA DANCR-miR-758-3p-PAX6 Molecular Network Regulates Apoptosis and Autophagy of Breast Cancer Cells

Authors Zhang XH, Li BF, Ding J, Shi L, Ren HM, Liu K, Huang CC, Ma FX, Wu XY

Received 15 March 2020

Accepted for publication 6 May 2020

Published 29 May 2020 Volume 2020:12 Pages 4073—4084

DOI https://doi.org/10.2147/CMAR.S254069

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Xian Hu Zhang,1 Bing Feng Li,1 Jie Ding,2 Lei Shi,1 Huo Ming Ren,1 Kui Liu,1 Chuan Cai Huang,1 Fu Xiao Ma,1 Xin Yao Wu1

1Department of General Surgery, Suzhou First People’s Hospital, Suzhou, Anhui 234000, People’s Republic of China; 2Physical Examination Department, Suzhou Central Blood Station, Suzhou, Anhui 234000, People’s Republic of China

Correspondence: Xian Hu Zhang
Department of General Surgery, Suzhou First People’s Hospital, No. 26 Yinhe Road, Suzhou, Anhui 234000, People’s Republic of China
Tel +86 18134605661
Email zhang64332277735@126.com

Objective: This study set out to probe into the effects of long non-coding RNA (LncRNA) differentiation antagonizing non-protein coding RNA (DANCR) on apoptosis and autophagy of breast cancer (BC) cells.
Methods: The expression levels of DANCR, miR-758-3p and paired box 6 (PAX6) in BC tissues and cell lines were detected. The transcription and protein levels of PAX6, apoptosis-related factors (caspase-3, caspase-9, Bax/Bcl-2), and autophagy-related factors (LC3B, Atg5, Beclin-1) in BC cells were detected. The cell proliferation, apoptosis, autophagy and the regulatory relationship between genes and target genes were analyzed.
Results: DANCR and PAX6 were up-regulated in BC tissues and cell lines, while miR-758-3p was opposite. Down-regulating DANCR inhibited the malignant proliferation of BC cells and also promoted apoptosis and autophagy, which showed that caspase-3, caspase-9, Bax/Bcl-2, LC3B, Atg5 transcription and protein levels increased, while Beclin-1 transcription and protein levels decreased. DANCR regulated miR-758-3p in a targeted manner, and its over-expression could weaken the anti-cancer effect of miR-758-3p on BC cells. In addition, miR-758-3p also directly targeted PAX6, and knocking down its expression could weaken the inhibitory effect of down-regulating PAK6 on BC cell apoptosis and autophagy. We also found that DANCR acted as a competitive endogenous RNA sponge miR-758-3p, thus regulating the PAX6 expression.
Conclusion: DANCR-miR-758-3p-PAX6 molecular network plays a key regulatory role in BC cell apoptosis and autophagy, which may provide reference for treating patients.

Keywords: DANCR, miR-758-3p, PAX6, breast cancer, BC, apoptosis and autophagy

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