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LINC00665 Stimulates Breast Cancer Progression via Regulating miR-551b-5p

Authors Qi L, Sun B, Yang B, Lu S

Received 1 August 2020

Accepted for publication 18 November 2020

Published 5 February 2021 Volume 2021:13 Pages 1113—1121

DOI https://doi.org/10.2147/CMAR.S275096

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Seema Singh


Liqiang Qi,1 Bo Sun,2 Beibei Yang,2 Su Lu2

1Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, People’s Republic of China; 2The 2nd Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, People’s Republic of China

Correspondence: Liqiang Qi
Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People’s Republic of China
Tel +86 10-87787130
Email qiliqiang_bj@tom.com

Introduction: Long intergenic non-protein coding RNA 665 (LINC00665) has been revealed to contribute cancer progression in many cancer types including liver and gastric cancer. However, the roles of LINC00665 in breast cancer (BC) remain to be explored.
Methods: We explored LINC00665 expression in BC tissues and normal tissues at GEPIA. Then, its expression in BC cells (HCC-1937, MDA-MB-231, and MCF-7) and normal cells (MCF10A) was analyzed with qRT-PCR. In addition, the mechanisms of LINC00665 in BC were explored using bioinformatic analyses, luciferase activity reporter assay, RNA pull-down assay, and rescue experiments.
Results: We showed LINC00665 expression was significantly increased in both BC tissues and cells. The knockdown of LINC00625 significantly inhibits BC cell growth and promotes cell apoptosis in vitro, while the overexpression of LINC00625 has the opposite effects on BC progression. LINC00665 could affect BC progression via regulating miR-551b-5p.
Discussion: Taken together, our study showed that the LINC00665/miR-551b-5p axis was involved in the progression of BC.

Keywords: LINC00665, miR-551b-5p, breast cancer, progression

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