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Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

Authors Luo DD, Ruan SC, Liu AP, Kong XD, Lee IS, Chen C

Received 7 June 2018

Accepted for publication 14 August 2018

Published 9 October 2018 Volume 2018:13 Pages 6223—6233

DOI https://doi.org/10.2147/IJN.S176596

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 3

Editor who approved publication: Dr Linlin Sun


Dandan Luo,1 Shichao Ruan,1 Aiping Liu,2 Xiangdong Kong,3 In-Seop Lee,3,4 Cen Chen1,5

1College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 2Center for Optoelectronics Materials and Devices, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 3College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 4Institute of Natural Sciences, Yonsei University, Seoul 03722, Korea; 5Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, People’s Republic of China

Background: Functionalizing biomaterial substrates with biological signals shows promise in regulating neural stem cell (NSC) behaviors through mimicking cellular microenvironment. However, diverse methods for immobilizing biological molecules yields promising results but with many problems. Biomimetic apatite is an excellent carrier due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. Therefore, it may provide a promising alternative in regulating NSC behaviors.
Methods: Biomimetic apatite immobilized with the extracellular protein – laminin (LN) was prepared through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing LN. The amount of coprecipitated LN and their release kinetics were examined. The adhesion and proliferation behaviors of NSC on biomimetic apatite immobilized with LN were investigated.
Results: The coprecipitation approach provided well retention of LN within biomimetic apatite up to 28 days, and supported the adhesion and proliferation of NSCs without cytotoxicity. For long-term cultivation, NSCs formed neurosphere-like aggregates on non-functionalized biomimetic apatite. A monolayer of proliferated NSCs on biomimetic apatite with coprecipitated LN was observed and even more stable than the positive control of LN coated tissue-culture treated polystyrene (TCP).
Conclusion: The simple and reproducible method of coprecipitation suggests that biomimetic apatite is an ideal carrier to functionalize materials with biological molecules for neural-related applications.

Keywords: biomimetic apatite, neural stem cell, coprecipitation, adhesion, proliferation, laminin
 

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