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Irisin functions to inhibit malignant growth of human pancreatic cancer cells via downregulation of the PI3K/AKT signaling pathway

Authors Zhang D, Zhang P, Li L, Tang N, Huang F, Kong X, Tan X, Shi G

Received 2 May 2019

Accepted for publication 15 August 2019

Published 4 September 2019 Volume 2019:12 Pages 7243—7249

DOI https://doi.org/10.2147/OTT.S214260

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Geoffrey Pietersz


Deguo Zhang,1,2 Ping Zhang,3 Luan Li,2 Nan Tang,1,2 Fei Huang,2,4 Xianguo Kong,5 Xueying Tan,2,* Guangjun Shi,2,*

1Department of Hepatobiliary Surgery, The Affiliated Qingdao Chengyang District People’s Hospital of Qingdao University, Qingdao 266109, Shandong Province, People’s Republic of China; 2Department of Hepatobiliary Surgery, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266000, Shandong Province, People’s Republic of China; 3Department of Gynecology, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266000, Shandong Province, People’s Republic of China; 4Department of Gastrointestinal Hernia Surgery, Qianxinan People’s Hospital, Xingyi 562400, Guizhou Province, People’s Republic of China; 5Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University, Philadelphia 19107, PA, USA

*These authors contributed equally to this work

Correspondence: Xueying Tan; Guangjun Shi
Department of Hepatobiliary Surgery, The Affiliated Qingdao Municipal Hospital of Qingdao University, No 1, Jiao Zhou Road, Shibei District, Qingdao 266000, Shandong Province, People’s Republic of China
Tel +86 185 6269 2818; 
Tel +86 186 6167 5603
Email [email protected][email protected]

Introduction: Irisin is a newly identified cytokine that has gained increasing attention because of its potential therapeutic applications in metabolic diseases and human cancers. Recently, accumulating evidence indicates that irisin plays an important role in the development and metastasis of various tumors. The aim of this study was to evaluate the effects and underlying mechanisms of irisin on malignant growth of pancreatic cancer cells.
Materials and methods: The anti-proliferative effect of irisin was examined using the CCK-8 assay. Irisin-induced apoptosis was determined by the annexin V-FITC/PI staining assay. The effects of irisin on cell migration and invasion were assessed using the scratch-induced wound healing assay and transwell invasion assay, respectively. The expression and phosphorylation of signaling proteins were detected by Western blot analysis.
Results: Our results showed that irisin inhibited cell proliferation and induced apoptosis of pancreatic cancer cells in a dose-dependent manner. In addition, irisin decreased the migration and invasion of pancreatic cancer cells. Finally, Western blot analysis revealed that irisin downregulated the PI3K/AKT signaling pathway.
Conclusion: Our findings suggest that irisin is a novel therapeutic agent for pancreatic cancer.

Keywords: irisin, pancreatic cancer, cell growth, migration, apoptosis

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