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Investigation of Antibiotic Resistance, Serotype Distribution, and Genetic Characteristics of 164 Invasive Streptococcus pneumoniae from North China Between April 2016 and October 2017

Authors Zhao C, Xie Y, Zhang F, Wang Z, Yang S, Wang Q, Wang X, Li H, Chen H, Wang H

Received 9 April 2020

Accepted for publication 9 June 2020

Published 3 July 2020 Volume 2020:13 Pages 2117—2128

DOI https://doi.org/10.2147/IDR.S256663

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony


Chunjiang Zhao, Yuhao Xie, Feifei Zhang, Zhanwei Wang, Shuo Yang, Qi Wang, Xiaojuan Wang, Henan Li, Hongbin Chen, Hui Wang

Department of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of China

Correspondence: Hui Wang
Department of Clinical Laboratory, Peking University People’s Hospital, Beijing, People’s Republic of China
Email whuibj@163.com

Background: Streptococcus pneumoniae infections are the major cause of global morbidity and mortality among children and patients aged more than 65 years. This study aimed to investigate the antimicrobial resistance, bacterial serotype distribution, and genetic characteristics of invasive S. pneumoniae from different cities in North China.
Materials and Methods: A total of 164 invasive S. pneumoniae strains were collected from 8 hospitals in 5 regions of North China between April 2016 and October 2017. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. Capsular serotypes were identified using the Quellung reaction test. Molecular epidemiology was investigated using multilocus sequence typing.
Results: S. pneumoniae isolates were highly resistant to macrolides, clindamycin, and tetracycline in all age groups. The overall rate of resistance to penicillin was 56.7%. However, fluoroquinolones and vancomycin maintained excellent antimicrobial activities. The rate of resistance to β-lactam in strains isolated from children aged less than 18 years was significantly higher than that in strains from other age groups. The most prevalent serotypes were 14 (22.6%), 19F (16.5%), non-vaccine types (14.0%), 19A (9.8%), and 23F (9.1%). The coverage for PCV10 and PCV13 was 59.8% and 75.6%, respectively. The vaccine coverage rate was the highest among children aged less than 5 years. The proportion of penicillin-resistant isolates was higher among vaccine-covered strains compared with non-covered strains. S. pneumoniae showed considerable clonal dissemination, and ST876 (28, 17.1%), ST271 (22, 13.4%), ST81 (17, 10.4%) and ST320 (14, 8.5%) were the major STs.
Conclusion: All the 164 invasive S. pneumoniae isolates demonstrated high resistance to antibiotics. The coverage of S. pneumoniae vaccine was higher in children than in adults.

Keywords: antimicrobial resistance, multilocus sequence typing, serotyping, Streptococcus pneumoniae

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