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Intraoperative use of spectral-domain optical coherence tomography during Descemet’s stripping automated endothelial keratoplasty

Authors Sng C, Gimeno FL, Mehta JS, Myint Htoon H, Tan DT

Received 7 December 2011

Accepted for publication 29 December 2011

Published 23 March 2012 Volume 2012:6 Pages 479—486

DOI https://doi.org/10.2147/OPTH.S28971

Review by Single-blind

Peer reviewer comments 2


Chelvin CA Sng1,2, Federico Luengo Gimeno1, Jodhbir S Mehta1,2, Hla Myint Htoon1, Donald T Tan1,2

1Singapore Eye Research Institute and Singapore National Eye Center, 2Department of Ophthalmology, National University Health System, Singapore

Purpose: To evaluate the intraoperative changes in the donor lenticule, recipient cornea, and the reduction of interface fluid thickness during Descemet’s stripping and automated endothelial keratoplasty with EndoGlide™ (Angiotech Pharmaceuticals Inc, Vancouver, Canada) donor insertion, using intraoperative spectral-domain optical coherence tomography.
Methods: Prospective observational case series of patients underwent Descemet’s stripping and automated endothelial keratoplasty using the EndoGlide inserter. Spectral-domain optical coherence tomography (iVue; Optovue Inc, Fremont, CA) with a handheld probe was used to image the cornea and anterior chamber. Standardized software was used to measure interface fluid gap, host cornea, and donor lenticule thicknesses during the following surgical stages of Descemet’s stripping and automated endothelial keratoplasty: (1) after donor insertion and immediately before full air tamponade; (2) after air tamponade and expression of fluid from venting incisions; (3) at 6 minutes of air tamponade; and (4) at 10 minutes of air tamponade.
Results: Ten patients with a mean age of 74.9 ± 11.8 years were recruited. Spectral-domain optical coherence tomography measurements of the interface fluid gap after fluid was expressed through the venting incisions (P < 0.001), at 6 minutes of air tamponade (P < 0.001) and at 10 minutes of air tamponade (P < 0.001 and P = 0.001, respectively), were significantly decreased compared to the measurements immediately before air tamponade. Donor thickness increased significantly at 6 minutes of air tamponade (P = 0.004) but reduced by 10 minutes compared to immediately before air tamponade.
Conclusion: Significant intraoperative changes in the donor, recipient cornea, and interface fluid thickness occurred following endothelial keratoplasty donor insertion.

Keywords: Descemet’s stripping and automated endothelial keratoplasty, spectral-domain optical coherence tomography

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