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Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles

Authors Rathinaraj P, Al-Jumaily AM, Huh DS

Received 14 September 2014

Accepted for publication 21 October 2014

Published 10 February 2015 Volume 2015:7 Pages 51—58

DOI https://doi.org/10.2147/BCTT.S69834

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Professor Pranela Rameshwar

Pierson Rathinaraj,1 Ahmed M Al-Jumaily,1 Do Sung Huh2

1Institute of Biomedical Technologies, Auckland University of Technology, Auckland, New Zealand; 2Department of Nano science and Engineering, Inje University, Gimhea, South Korea


Abstract: Herceptin, the monoclonal antibody, was successfully immobilized on gold nanoparticles (GNPs) to improve their precise interactions with breast cancer cells (SK-BR3). The mean size of the GNPs (29 nm), as determined by dynamic light scattering, enlarged to 82 nm after herceptin immobilization. The in vitro cell culture experiment indicated that human skin cells (FB) proliferated well in the presence of herceptin-conjugated GNP (GNP–Her), while most of the breast cancer cells (SK-BR3) had died. To elucidate the mechanism of cell death, the interaction of breast cancer cells with GNP–Her was tracked by confocal laser scanning microscopy. Consequently, GNP–Her was found to be bound precisely to the membrane of the breast cancer cell, which became almost saturated after 6 hours incubation. This shows that the progression signal of SK-BR3 cells is retarded completely by the precise binding of antibody to the human epidermal growth factor receptor 2 receptor of the breast cancer cell membrane, causing cell death.

Keywords: herceptin, gold nanoparticles, SK-BR3 cells, intracellular uptake

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