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Initial experience of correlating diffusion spectral parameters with histopathologic indexes in murine colorectal tumor homografts

Authors Zhang XY, Li XT, Sun J, Sun YS

Received 10 November 2016

Accepted for publication 16 May 2017

Published 26 August 2017 Volume 2017:10 Pages 4213—4223

DOI https://doi.org/10.2147/OTT.S127283

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Dr Carlos Vigil Gonzales

Xiao-Yan Zhang, Xiao-Ting Li, Jia Sun, Ying-Shi Sun

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital & Institute, Beijing, People’s Republic of China

Purpose: To determine the correlation between continuously distributed diffusion-weighted image (DWI)-derived parameters and histopathologic indexes.
Methods: Fifty-four mice bearing HCT-116 colorectal tumors were included for analysis; 12 mice were used for continuous observation, and the other 42 mice were used for break-point observation. All mice were randomly divided into radiotherapy and non-radiotherapy groups. Optical imaging and MRI were performed at different time points according to radiotherapy regimen (baseline, 24 h, 48 h, 72 h, 7 d, 14 d, and 28 d). Continuous observation data were analyzed to show the difference of dynamic changing trends of optical and MR-DWI–derived parameters between radiotherapy and non-radiotherapy groups (photon numbers, D_max, full width half maximum [FWHM], and apparent diffusion coefficient [ADC] value). Break-point observation data were used to analyze the correlation between histopathologic indices and DWI-derived parameters.
Results: There was a significant difference in the changing trends of photon numbers, D_max, FWHM, and ADC value between radiotherapy and non-radiotherapy groups, especially at early time points. There was moderate negative correlation between Ki67 and percentage changes of D_max, FWHM, and ADC values (the correlation coefficients were 0.632, 0.449, and 0.586, P<0.001, P=0.008, and P<0.001, respectively). There was moderate negative correlation between survivin and percentage changes of D_max and ADC values (correlation coefficients were 0.496 and 0.473, P=0.004 and P=0.006, respectively).
Conclusion: The continuously distributed DWI-derived parameters could reflect histological behavior to some extent and, thus, are potential markers for early noninvasive monitoring of tumor cell apoptosis and proliferation.

Keywords: magnetic resonance imaging, diffusion-weighted imaging, continuously distributed, colorectal cancer, murine homografts

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