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Inhibitory Effect of Crocin Against Gastric Carcinoma via Regulating TPM4 Gene

Authors Luo Y, Yu P, Zhao J, Guo Q, Fan B, Diao Y, Jin Y, Wu J, Zhang C

Received 16 March 2020

Accepted for publication 21 November 2020

Published 7 January 2021 Volume 2021:14 Pages 111—122

DOI https://doi.org/10.2147/OTT.S254167

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Gaetano Romano


Yushuang Luo, Pengjie Yu, Junhui Zhao, Qijing Guo, Baohua Fan, Yinzhuo Diao, Yulong Jin, Jing Wu, Chengwu Zhang

Department of Oncology, Affiliated Hospital of Qinghai University, Xining 810001, People’s Republic of China

Correspondence: Chengwu Zhang
Department of Oncology, Affiliated Hospital of Qinghai University, Xining 810001, People’s Republic of China
Email cwzhang19@163.com

Background: Gastric cancer (GC) is one of the most common malignant tumors and the second most frequent cause of cancer death worldwide. Crocin is a kind of bioactive constituent found in the stigmas of saffron, which has shown various pharmacological activities.
Methods: In this study, we investigated the inhibitory effect of crocin on gastric cancer AGS cells proliferation and explored the underlying mechanism. A series of methods were used including cell counting kit assay, gene microarray analysis, qRT-PCR, Celigo image cytometry, cell clone formation assay, Western blot, and cell xenograft growth in vivo.
Results: The results indicated that crocin inhibited AGS cells proliferation and promoted cell apoptosis. Further studies suggested that crocin decreased a series of genes expression, among which TPM4 gene downregulation inhibited the tumor cells proliferation and tumor growth in mice, and overexpression of TPM4 gene abolishes the inhibitory effect of crocin. Further study using microarray analysis suggested that knocking down of TPM4 altered genes related to the proliferation and apoptosis of cells.
Discussion: Crocin could inhibit the gastric cancer cells AGS cells proliferation by regulating TPM4 gene expression, and TPM4 may be a promising therapeutic target for GC treatment.

Keywords: gastric cancer, crocin, TPM4, HER2

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