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Inhibition of XIAP increases carboplatin sensitivity in ovarian cancer

Authors Zhang Y, Huang F, Luo Q, Wu X, Liu Z, Chen H, Huang Y

Received 13 April 2018

Accepted for publication 2 October 2018

Published 5 December 2018 Volume 2018:11 Pages 8751—8759

DOI https://doi.org/10.2147/OTT.S171053

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Geoffrey Pietersz


Yiping Zhang,1,2 Furong Huang,3 Qingyu Luo,3 Xiaowei Wu,3 Zhihua Liu,3 Hongyan Chen,3 Yinghui Huang1

1Cancer Institute, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China; 2China National Center for Biotechnology Development, Beijing, China; 3The State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

Purpose: Carboplatin is a first-line treatment for ovarian cancer. However, most patients develop resistance and undergo disease recurrence. This study aims to explore the relationship between the expression of X-linked inhibitor of apoptosis protein (XIAP) and carboplatin sensitivity in ovarian cancer.
Patients and methods: We examined the expression of XIAP in ovarian cancer by immunochemistry. Next, we investigated the role of XIAP in regulating carboplatin sensitivity in ovarian cancer ES2 and 3AO cells through Cell Counting Kit-8 cell viability assay and fluorescein isothiocyanate-Annexin V/propidium iodide apoptosis assay. Expression of apoptotic effectors was measured by Western blot.
Results: The immunochemistry results showed that high XIAP expression levels inversely correlated with carboplatin response (P=0.03) and progression-free survival (P=0.0068) in patients with ovarian cancer. Knockdown of XIAP repressed the cell viabilities in the carboplatin-treated cells and increased carboplatin-induced caspase activation. In summary, our data show that XIAP mediates carboplatin sensitivity of ovarian cancer.
Conclusion: In summary, our data show that XIAP mediates carboplatin sensitivity of ovarian cancer and XIAP may be a novel target for the treatment of carboplatin-resistant ovarian cancer.

Keywords: XIAP, carboplatin, chemosensitivity, ovarian cancer

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