Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Inhibition of lung cancer cells A549 and H460 by curcuminoid extracts and nanoemulsions prepared from Curcuma longa Linnaeus

Authors Chang HB, Chen BH

Received 23 April 2015

Accepted for publication 13 June 2015

Published 6 August 2015 Volume 2015:10(1) Pages 5059—5080

DOI https://doi.org/10.2147/IJN.S87225

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster

Hong-Bin Chang,1 Bing-Huei Chen1,2

1
Department of Food Science, 2Graduate Institute of Medicine, Fu Jen Catholic University, Taipei, Taiwan

Abstract: The objectives of this study were to explore the inhibition mechanism of lung cancer cells A549 and H460 by curcuminoid extracts and nanoemulsions prepared from Curcuma longa Linnaeus. In addition, human bronchus epithelial cell line BEAS-2B (normal cell) was selected for comparison. A high-performance liquid chromatography (HPLC) method was developed to separate and quantify the various curcuminoids in C. longa extract, including curcumin (1,714.5 µg/mL), demethoxycurcumin (1,147.4 µg/mL), and bisdemethoxycurcumin (190.2 µg/mL). A high-stability nanoemulsion composed of Tween 80, water, and curcuminoid extract was prepared, with mean particle size being 12.6 nm. The cell cycle was retarded at G2/M for both the curcuminoid extract and nanoemulsion treatments; however, the inhibition pathway may be different. H460 cells were more susceptible to apoptosis than A549 cells for both curcuminoid extract and nanoemulsion treatments. Growth of BEAS-2B remained unaffected for both the curcuminoid extract and nanoemulsion treatments, with a concentration range from 1 to 4 µg/mL. Also, the activities of caspase-3, caspase-8, and caspase-9 followed a dose-dependent increase for both A549 and H460 cells for both the treatments, accompanied by a dose-dependent increase in cytochrome C expression and a dose-dependent decrease in CDK1 expression. Interestingly, a dose-dependent increase in cyclin B expression was shown for A549 cells for both the treatments, while a reversed trend was found for H460 cells. Both mitochondria and death receptor pathways may be responsible for apoptosis of both A549 and H460 cells.

Keywords: curcuminoid extract, curcuminoid nanoemulsion, Curcuma longa Linnaeus, lung cancer cell, cell cycle, apoptosis mechanism

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Readers of this article also read:

Real-time analysis of dual-display phage immobilization and autoantibody screening using quartz crystal microbalance with dissipation monitoring

Rajaram K, Losada-Pérez P, Vermeeren V, Hosseinkhani B, Wagner P, Somers V, Michiels L

International Journal of Nanomedicine 2015, 10:5237-5247

Published Date: 19 August 2015

Nanotechnology for treating osteoporotic vertebral fractures

Gao C, Wei D, Yang H, Chen T, Yang L

International Journal of Nanomedicine 2015, 10:5139-5157

Published Date: 13 August 2015

Pharmacological characterization of nanoparticle-induced platelet microaggregation using quartz crystal microbalance with dissipation: comparison with light aggregometry

Santos-Martinez MJ, Tomaszewski KA, Medina C, Bazou D, Gilmer JF, Radomski MW

International Journal of Nanomedicine 2015, 10:5107-5119

Published Date: 13 August 2015

Lactoferrin-modified PEGylated liposomes loaded with doxorubicin for targeting delivery to hepatocellular carcinoma

Wei MY, Guo XC, Tu LX, Zou Q, Li Q, Tang CY, Chen B, Xu YH, Wu CB

International Journal of Nanomedicine 2015, 10:5123-5137

Published Date: 12 August 2015

Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.

Bonifácio BV, Ramos MAS, Silva PB, Negri KMS, Lopes EO, Souza LP, Vilegas W, Pavan FR, Chorilli M, Bauab TM

International Journal of Nanomedicine 2015, 10:5081-5092

Published Date: 10 August 2015

pH-Responsive therapeutic solid lipid nanoparticles for reducing P-glycoprotein-mediated drug efflux of multidrug resistant cancer cells

Chen HH, Huang WC, Chiang WH, Liu TI, Shen MY, Hsu YH, Lin SC, Chiu HC

International Journal of Nanomedicine 2015, 10:5035-5048

Published Date: 5 August 2015

Nanotechnology-based drug delivery systems for the treatment of Alzheimer’s disease

Fonseca-Santos B, Gremião MP, Chorilli M

International Journal of Nanomedicine 2015, 10:4981-5003

Published Date: 4 August 2015

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Wang B, Tan L, Deng D, Lu T, Zhou C, Li Z, Tang Z, Wu Z, Tang H

International Journal of Nanomedicine 2015, 10:3417-3427

Published Date: 8 May 2015