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Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

Authors Lara-Cruz C, Jiménez-Salazar JE, Ramón-Gallegos E, Damian-Matsumura P, Batina N

Received 18 March 2016

Accepted for publication 10 June 2016

Published 7 October 2016 Volume 2016:11 Pages 5149—5161


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Thomas Webster

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C Lara-Cruz,1 JE Jiménez-Salazar,1 E Ramón-Gallegos,2 P Damian-Matsumura,1 N Batina3

1Department of Biology of Reproduction, Metropolitan Autonomous University, 2Department of Morphology, National School of Biological Sciences, National Polytechnic Institute, 3Department of Chemistry, Nanotechnology and Molecular Engineering Laboratory, Metropolitan Autonomous University, Mexico City, Mexico

Abstract: Gold nanoparticles (AuNPs) have been proposed for use in the treatment of different types of cancer, including breast cancer. At present, neither the mechanisms of AuNP interaction with the plasma membrane surface and their delivery and intracellular distribution in cancer cells nor their effect on the plasma membrane so as to allow cell incorporation of larger amounts of AuNPs is known. The objective of this work was to study the interaction of bare 20 nm diameter AuNPs with the plasma membrane of human MCF-7 breast cancer cells, as well as their uptake, intracellular distribution, and induction of changes on the cell surface roughness. The dynamics of intracellular incorporation and the distribution of AuNPs were observed by confocal laser scanning microscopy. Changes in roughness were monitored in synchronized MCF-7 cells by atomic force microscopy high-resolution imaging at 6 hour intervals for 24 hours during a single cell cycle. The results show that bare AuNPs are capable of emitting fluorescence at 626 nm, without the need for a fluorescent biomarker, which allows monitoring their uptake and intracellular distribution until they reach the nucleus. These results are correlated with changes in cell roughness, which significantly increases at 12 hours of incubation with AuNPs, when compared with control cells. The obtained data provide bases to understand molecular processes of the use of AuNPs in the treatment of different diseases, mainly breast cancer.

gold nanoparticles uptake, MCF-7 cells, membrane roughness, atomic force microscopy, confocal laser scanning microscopy

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