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Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation

Authors Genier FS, Bizanek M, Webster TJ, Roy AK

Received 5 August 2017

Accepted for publication 9 October 2017

Published 9 February 2018 Volume 2018:13 Pages 895—901

DOI https://doi.org/10.2147/IJN.S148390

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Alexander Kharlamov

Peer reviewer comments 2

Editor who approved publication: Professor Carlos Rinaldi


Francielli S Genier,1 Maximilian Bizanek,1 Thomas J Webster,1,2 Amit K Roy1,2

1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Wenzhou Institute of Biomaterials and Engineering (WIBE), Wenzhou University, Wenzhou, People’s Republic of China

Abstract: Conditions of cellular stress are often the cause of cell death or dysfunction. Sustained cell stress can lead to several health complications, such as extensive inflammatory responses, tumor growth, and necrosis. To prevent disease and protect human tissue during these conditions and to avoid medication side effects, nanomaterials with unique characteristics have been applied to biological systems. This paper introduces the pretreatment in human dermal fibroblasts with cerium oxide nanoparticles during nutritional stress. For this purpose, human dermal fibroblast cells received cell culture media with concentrations of 250 µg/mL and 500 µg/mL of nano-cerium oxide before being exposed to 24, 48, and 72 hours of serum starvation. Contrast images demonstrated higher cell confluence and cell integrity in cells pretreated with ceria nanoparticles compared to untreated cells. It was confirmed by MTS assay after 72 hours of serum starvation that higher cell viability was achieved with ceria nanoparticles. The results demonstrate the potential of cerium oxide nanoparticles as protective agents during cellular starvation.

Keywords: cerium oxide, nanoparticles, serum starvation, human dermal fibroblasts

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