In vitro safety and performance evaluation of a seawater solution enriched with copper, hyaluronic acid, and eucalyptus for nasal lavage
Received 25 March 2019
Accepted for publication 20 May 2019
Published 24 September 2019 Volume 2019:12 Pages 399—410
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Ms Justinn Cochran
Peer reviewer comments 2
Editor who approved publication: Dr Scott Fraser
Song Huang,1 Samuel Constant,1 Barbara De Servi,2 Marisa Meloni,2 Josip Culig,3 Marco Bertini,4 Amina Saaid5
1Epithelix, Geneva, Switzerland; 2Department of in Vitro Research, VitroScreen, Milan, 20149, Italy; 3Department of Pharmacology, University of Applied Health Sciences, Zagreb, 10000, Croatia; 4R&D Department, Laboratori Baldacci SpA, Pisa, Italy; 5Department of R&D and Innovation, Laboratoire Fumouze, Levallois-Perret, 92686, France
Correspondence: Marco Bertini
Laboratori Baldacci SpA, Via S. Michele degli Scalzi, 73, 56124 Pisa, Italy
Tel +39 5 031 3271
Fax +39 5 057 0170
Background: The common cold is a viral infectious disease with symptoms such as runny nose, sore throat, and mainly, nasal congestion. State-of-the-art therapeutic approaches focus on alleviating the symptoms of this disease by non-invasive and simple-to-use methods. Nasal irrigation is one of the most accepted approaches to ease nasal congestion which, if left untreated, has a negative impact on the quality of life of patients.
Purpose: In this study, the safety and efficacy of a novel hypertonic seawater solution for nasal lavage enriched with hyaluronic acids, eucalyptus oil, copper, and manganese salts (Stérimar Stop & Protect Cold and Flu; SSPCF) have been investigated in vitro.
Methods: An in vitro 3D reconstituted human nasal epithelium tissue model, MucilAir™, has been used in this study to investigate the safety of SSPCF on nasal epithelium by measuring transepithelial electrical resistance (TEER), lactate dehydrogenase (LDH), and interleukin-8 (IL-8) secretion. The efficacy of SSPCF was measured by mucociliary clearance (MCC), ATP release, Alcian blue and aquaporin (AQP3) stainings.
Results: SSPCF treatment respected nasal epithelium tissue integrity and enhanced barrier function without inducing a cytotoxic response. Secreted LDH and IL-8 levels were similar to untreated controls. MCC rate was increased 2.5-fold and ATP release decreased 87% upon SSPCF treatment, indicating improved decongestion activity. SSPCF treatment after hypotonic stress helped recover cellular organization, as shown by Alcian blue and AQP3 staining assays.
Conclusion: SSPCF appears as a safe and effective nasal irrigation formula that may alleviate the symptoms associated with common cold such as nasal congestion.
Keywords: common cold, hypertonic seawater, nasal irrigation, pathogens, ATP release, decongestion
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