In vitro anticoagulant effect of Crassocephalum crepidioides leaf methanol extract and fractions on human blood
Received 5 June 2019
Accepted for publication 30 July 2019
Published 4 September 2019 Volume 2019:11 Pages 99—107
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Professor Bal Lokeshwar
Opeyemi Oluwayemisi Ayodele,1 Funmilayo Dorcas Onajobi,1 Omolaja Osoniyi1,2
1Department of Biochemistry, College of Health and Medical Science, Benjamin Carson (Snr.) School of Medicine, Babcock University, Ibadan, Nigeria; 2Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria
Correspondence: Opeyemi Oluwayemisi Ayodele
Department of Biochemistry, Benjamin Carson (Snr.) School of Medicine, Babcock University, PMB 4003, Ilishan-Remo, Ogun State, Nigeria
Tel +234 806 620 0610
Background: Blood coagulation is a rapid and efficient process that produces clot formation which requires regulation. A derangement of blood coagulation is a feature of many disease conditions. This study investigated the in vitro effects of Crassocephalum crepidioides Benth S. Moore leaf methanol (crude) extract and its partitioned solvent fractions on blood coagulation of Healthy human volunteers.
Methods: The secondary metabolites from dried and ground C. crepidioides leaves were extracted with 70% methanol, and the concentrated crude extract was subsequently subjected to solvent partitioning with Hexane, Ethyl acetate, and Butanol. Varying concentrations (5–20 mg/mL) of the extract and fractions were tested in vitro on blood coagulation profile; clotting time (CT), prothrombin time (PT), and activated partial thromboplastin time (aPTT) of apparently healthy human volunteers, while phytochemical characterization of the Hexane fraction was done by gas chromatography-mass spectrometry (GC-MS).
Results: C. crepidioides leaf methanol extract and fractions significantly (P<0.05) prolonged the clotting time, prothrombin and activated partial thromboplastin times in the blood obtained from the volunteers. The highest prolongation effect was recorded with the Hexane fraction at concentration of 10mg/mL. GC-MS analysis of the Hexane fraction indicated the presence of phytochemicals such as unsaturated fatty acids and esters, phenolic compounds, flavonoids, and coumarin-related compounds known to exhibit antiaggregant, antiplatelet and antimicrobial activities.
Conclusion: These results showed that C. crepidioides possesses bioactive components with anticoagulant properties which may be exploited in the treatment of blood coagulation disorders.
Keywords: blood coagulation, clotting time, prothrombin time, solvent partitioning, Crassocephalum crepidioides
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