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In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers

Authors Narita T, Yunoki S, Ohyabu Y, Yahagi N, Uraoka T

Received 6 July 2016

Accepted for publication 6 September 2016

Published 15 December 2016 Volume 2016:9 Pages 429—439

DOI https://doi.org/10.2147/MDER.S116633

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Akshita Wason

Peer reviewer comments 2

Editor who approved publication: Dr Scott Fraser

Takefumi Narita,1 Shunji Yunoki,1 Yoshimi Ohyabu,1 Naohisa Yahagi,2 Toshio Uraoka3

1Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku, 2Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Shinjuku-ku, 3Department of Gastroenterology, National Hospital Organization Tokyo Medical Center, Meguro-ku, Tokyo, Japan

Abstract: We investigated the potential of collagen–genipin sols as biomaterials for treating artificial ulcers following endoscopic submucosal dissection. Collagen sol viscosity increased with condensation, allowing retention on tilted ulcers before gelation and resulting in collagen gel deposition on whole ulcers. The 1.44% collagen sols containing genipin as a crosslinker retained sol fluidity at 23°C for >20 min, facilitating endoscopic use. Collagen sols formed gel depositions on artificial ulcers in response to body temperature, and high temperature responsiveness of gelation because of increased neutral phosphate buffer concentration allowed for thick gel deposition on tilted ulcers. Finally, histological observations showed infiltration of gels into submucosal layers. Taken together, the present data show that genipin-induced crosslinking significantly improves the mechanical properties of collagen gels even at low genipin concentrations of 0.2–1 mM, warranting the use of in situ gelling collagen–genipin sols for endoscopic treatments of gastrointestinal ulcers.

Keywords: collagen, genipin, in-situ gel, fibril formation, ulcer dressing
 

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